Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (7): 1622.

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Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin

WANG Lin¹, LI Chun-Yan¹, HE Pan², FU Li¹, ZHOU Yan-Min^1*, CHEN Xue-Si²

1. Implant Center, Stomatological Hospital of Jilin University, Changchun 130021, China;
2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2010-11-11 Revised:2011-03-04 Online:2011-07-10 Published:2011-06-02
Contact: Yan-Min ZHOU E-mail:jlcyspring@126.com
Supported by:
口腔疾病研究国家重点实验室创新人才培养课题基金(批准号: SKLODd2009001)及吉林省产业技术研究与开发项目(批准号: 20101935)资助.

Abstract

Abstract: In the presence of tripolyphosphate ( TPP) ,chitosan microspheres (CMs) loaded with adrenomedullin(ADM) were prepared by an emulsion-ionic cross-linking method .Then, Porous CMs/ nano-hydroxyapatite/ Poly(Lactide-co-Glycolide) composite was developed by thermally induced phase separation. Scanning electron microscope was used to analyze the characterization of the microspheres and scaffold. In vitro the drug release from microspheres and scaffold have been tested, also the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. The MG63 cell line and HUVEC cell line were cultured for testing the bioactivity of the scaffold loading ADM. The cell proliferation and differentiation was analyzed by MTT assay and ALP assay The result demonstrated the diameter of the microspere was well-distributed. The nHAC/PLGA/ADM scaffold showed a typical microstructure of polymeric foam prepared by thermally induced phase separation. The pore size ranged from 30μm to 220μm.and and the pores were interconnected.The hemolytic rate of the biomaterial was less than 5% ,which was in line with national standard of medical materials.The nHAC/PLGA/ADM and nHAC/PLGA scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells, also were the stimuli for the differentiation of osteoblast-like cells.

Key words: Chitosan, microspheres, Poly(Lactide-co-Glycolide)(PLGA), nano-hydroxyapatite, adrenomedullin

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WANG Lin, LI Chun-Yan, HE Pan, FU Li, ZHOU Yan-Min*, CHEN Xue-Si. Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin[J]. Chem. J. Chinese Universities, 2011, 32(7): 1622.

References

[1]Kitamara K,Kangawa K,Kawamoto M,et al.Biochem Biophys Res Commun[J],1993, 192(2):553-560. [2] Hinson JP, Kapas S, Smith DM. Endocr Rev[J],2000,21:138– 167. [3]Ichiki Y, Kitamura K, Kangawa K, et al. FEBS Lett[J],1994;338(1):6-10. [4]J. Cornish, D. Naot, I.R. Reid. Regul Pept [J],2003,112:79– 86. [5] Domenico Ribatti , Beatrice Nico, Raffaella Spinazzi,et al. Peptides[J],2005,26:1670–1675. [6]Allaker RP, Kapas S. Regul Pept[J],2003,112:147-152. [7]Michael Gr?schl, Olaf Wendler ,Hans-Georg Topf,et al. Regul Pept[J],2009,154:16-22. [8]Zhang Y, Zhang MQ. J Biomed Mater Res[J].2002,62(3): 378-386. [9]Kumar G,Bristow JF,Smith PJ,et al. Ploymer[J],2000,41(6): 2157-2168. [10]Varshosaz J. Expert Opin[J],2007,4:263–273. [11]Desai KGH, Park HJ. J Microencapsulation[J],2005a,22:179–192. [12] LI Jun-Feng(李峻峰),ZHANG Li(张利),LI Jun-Fu(李钧甫),et al. Chem. J. Chinese Universities(高等学校化学学报) [J], 2008, 29 (9) :1874-1879. [13] S.E. Kim, J.H. Park, Y.W. Cho, et al. Journal of Controlled Release[J],2003,91 (3):365-374. [14] X.Z. Shu, K.J. Zhu. International Journal of Pharmaceutics[J] ,2002,233 (1-2) :217-225. [15]Desai KG, Liu C, Park HJ. J Microencapsulation[J],2006,23:79-90. [16]Cai DZ, Zeng C, Quan DP, et al. Chin MeD J[J],2007;120:197-203. [17]Sunil A. Agnihotri, et al. Journal of Controlled Release[J], 2004,100 (5) :5-28 [18]Xufeng Niu,Qingling Feng,et al. Journal of Controlled Release[J],2009,134:111-117 [19] Lin CY, Schek RM, Mistry AS, et al.Tissue Eng[J], 2005, 11(9-10): 1589-1598. [20]Xufeng Niu, Qingling Feng, et al. Journal of Microencapsulation[J],2009,26(4): 297–305 [21]Ko JA, Park HJ, Hwang SJ, et al. Int J Pharm[J],2002;249:165–174. [22] Joy Patricia Hinson, Supriya Kapas, David Michael Smith. Endocrine Reviews[J],2000, 21(2): 138–167. [23]Chen FM,Wu ZF,Sun HH,et al. Int J Pharm[J],2006,307:23–32. [24]Xufeng Niu,Qingling Feng,Mingbo Wang,et al.Polymer Degradation and Stability[J],2009, 94: 176-182. [25]Jillian Cornish,Karen E.Callon,Dacid H.Coy, et al. Am J Physiol[J], 1997,273(6Pt1): E1113 -1120 [26] Buhlmann N, Leuthauser K, Muff R, et al. Endocrinology[J],1999,140:2883–2890. [27]Leonid L. Nikitenko, David M. Smith, Stephen Hague. Trends in Pharmacological Sciences[J], 2002,23:101-103. [28] Diego Guidolin , Giovanna Albertin ,Raffaella Spinazzi,et al. Peptides[J],2008,29:2013-2023. [29]Anselme K.Biomaterials[J],2000,21:667-681 [30]Dan Ferber. Science,1999,284(5413):489-493. [31]YU Ting(于婷), LIU Ya(刘娅),WANG Yu(王宇), et al. Chem. J. Chinese Universities(高等学校化学学报) [J], 2009, 30 (7) :1439-1444.

Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin

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