Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (11): 2419.doi: 10.7503/cjcu20190193

• Polymer Chemistry • Previous Articles    

Silk Fibroin-modified Ploylactic Acid-glycolic Acid Copolymer Porous Microspheres as Gingival Mesenchymal Stem Cells Delivery Carrier

LIU Yaoshan1,WU Hailin1,JIA Zhi1,DU Bo2,LIU Dayong1,*(),ZHOU Zhimin2,*()   

  1. 1. Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
    2. Tianjin Key Laboratory of Biomedical Materials, Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China
  • Received:2019-04-02 Online:2019-11-10 Published:2019-10-15
  • Contact: LIU Dayong,ZHOU Zhimin E-mail:dyliuperio@tmu.edu.cn;zhouzm@bme.cams.cn
  • Supported by:
    ? Supported by the National Natural Science Foundation of China(81371109);? Supported by the National Natural Science Foundation of China(81670953);the CAMS Innovation Fund for Medical Sciences, China(2017-I2M-1-007);the Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Human Resources and Social Security, China(201519205)

Abstract:

The polylactic acid-glycolic acid copolymer(PLGA) porous microspheres suitable for three-dimensional cell culture were prepared by double emulsion-solvent evaporation method, which were then subjected to silk fibroin modification, and characterized by scanning electron microscopy, energy dispersive spectroscopy, infrared spectroscopy and X-ray diffraction. Primary cultured human gingival mesenchymal stem cells were used for osteogenic(alizarin red staining) and adipogenic(oil red O staining) differentiation identification experiments. The gingival stem cells were seeded on silk fibroin-modified PLGA porous microspheres by negative pressure suspension method, and the 5-ethynyl-2'-deoxyuridine(EdU) cell proliferation experiment and osteogenic differentiation experiment were performed. The result confirmed that primary cultured gingival stem cells had multi-directional differentiation potential, and those loaded on silk fibroin-modified PLGA porous microspheres were beneficial to cell proliferation. Silk fibroin modified PLGA porous microspheres can be good cell delivery carriers, providing a scientific basis for further repair of alveolar bone defects.

Key words: Gingival mesenchymal stem cell, Polylactic acid-glycolic acid copolymer porous microspheres, Silk fibroin-modification, Tissue repairing

CLC Number: 

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