Preparation of Yolk-shell Fe3O4@SiO2@PMO Magnetic Microspheres for Laccase Immobilization†

doi:10.7503/cjcu20180517

Abstract

Abstract:

Yolk-shell Fe₃O₄@SiO₂@PMO magnetic microspheres were prepared and used to immobilize laccase using glutaraldehyde as the crosslinking agent. The influence of some experimental factors, such as glutaraldehyde concentration, crosslinking time and immobilization time, on laccase immobilization were investigated. The largest immobilization amount of laccase in the Fe₃O₄@SiO₂@PMO microspheres is 475 mg/g. After immobilization, the laccase could survive in harsh environment, showing relative enzyme activities of 70% and 65% at pH=2.5—4.5 and 60 ℃, respectively. This means immobilization on Fe₃O₄@SiO₂@PMO could afford higher stability than bare laccase. Besides, immobilized laccase could be recycled easily via simply magnetic separation, with activity retention of 85% even after 10 times recycle.

Key words: Periodic mesoporous hybrid inorganic-organic silica, Yolk-shell magnetic microspheres, Laccase, Immobilization

CLC Number:

TrendMD:

DUAN Bingyi,WANG Yu,GUO Ningning,WANG Runwei,ZHANG Zongtao,QIU Shilun. Preparation of Yolk-shell Fe₃O₄@SiO₂@PMO Magnetic Microspheres for Laccase Immobilization^†[J]. Chem. J. Chinese Universities, 2019, 40(2): 210.

Figures/Tables 7

Fig.1 TEM images of yolk-shell Fe3O4@SiO2@PMO microspheres(A, B) and Fe3O4@SiO2@PMO microspheres-laccase(C, D)

Fig.2 Wide-angle XRD patterns(A) and magnetic hysteresis loops recorded at 25 ℃ of Fe3O4(a), Fe3O4@SiO2(b) and yolk-shell Fe3O4@SiO2@PMO(c)(B)Inset of (B): yolk-shell Fe3O4@SiO2@PMO samples without(a) and with(b) magnet.

Fig.3 N2 adsorption-desorption isotherms(A) and pore size distribution(B) of yolk-shell Fe3O4@SiO2@PMO microspheres(a) and Fe3O4@SiO2@PMO microspheres-laccase(b)

Fig.4 Effect of glutaraldehyde concentration on immobilization amount and activity of laccase

Fig.5 Effect of crosslinking time(A) and immobilization time(B) on immobilization amount of laccase

Fig.6 pH stabilities(A) and temperature stabilities(B) of free laccase(a) and Fe3O4@SiO2@PMO microspheres immobilized laccase(b)

Fig.7 Lineweaver-Burk plot of free laccase(a) and Fe3O4@SiO2@PMO microspheres immobilized laccase(b)(A), and operational stability of Fe3O4@SiO2@PMO microspheres immobilized laccase(B)

References 26

[1]	Thurston C. F., Microbiology, 1994, 140(1), 19-26
[2]	Eichlerova I., Snajdr J., Baldrian P., Chemosphere,2012, 88(10), 1154-1160
[3]	Fernández-Fernández M., Sanromán M., Moldes D., Biotechnol. Adv.,2013, 31(8), 1808-1825
[4]	Pang R., Li M., Zhang C., Talanta,2015, 131, 38-45
[5]	Godoy-Navajas J., Aguilar-Caballos M. P., Gómez-Hens A., Food Chem., 2015, 166, 29-34
[6]	Moreira S., Milagres A. M. F., Mussatto S. I., Sep. Purif. Technol., 2014, 135, 183-189
[7]	Couto S. R., Herrera J. L. T., Biotechnol. Adv.,2006, 24(5), 500-513
[8]	Verma M. L., Puri M., Barrow C. J., Crit. Rev. Biotechnol.,2016, 36(1), 108-119
[9]	Li Z., Barnes J. C., Bosoy A., Stoddart J. F., Zink J. I., Chem. Soc. Rev.,2012, 41(7), 2590-2605
[10]	Forde J., Tully E., Vakurov A., Gibson T. D., Millner P., Ó’Fágáin C., Enzyme Microb. Technol., 2010, 46(6), 430-437
[11]	Ispas C., Sokolov I., Andreescu S., Ana. Bioanal. Chem.,2009, 393(2), 543-554
[12]	Zhu Y. F., Shi J. L., Shen W. H., Chen H. R., Dong X. P., Ruan M. L., Nano. Technol.,2005, 16(11), 2633-2638
[13]	Salis A., Meloni D., Ligas S., Casula M. F., Monduzzi M., Solinas V., Dumitriu E., Langmuir,2005, 21(12), 5511-5516
[14]	Xu X. H., Lu P., Zhou Y. M., Zhao Z. Z., Guo M. Q., Mater. Sci. Eng.,2009, 29(7), 2160-2164
[15]	Xie W., Zang X., Food Chem.,2016, 194, 1283-1292
[16]	Kalantari M., Kazeneini M., Arpanaei A., Biochem. Eng. J., 2013, 79, 267-273
[17]	Gibson L. T., Chem. Soc. Rev., 2014, 43(15), 5173-5182
[18]	Hoffmann F., Cornelius M., Morell J., Fröba M., Angew. Chem. Int. Ed., 2006, 45(20), 3216-3251
[19]	Wang J., Zhang W., Gu C., Zhang W., Zhou M., Wang Z., Guo C., Sun L., Chemistry-An Asian Journal,2017, 12(24), 3162-3171
[20]	Lan J. N., Na W., Wei Q., Li Q. Y., Wang W., Nie Z. R., Chem. J. Chinese Universities,2010, 31(8), 1579-1584
	(兰甲宁, 纳薇, 韦奇, 李群艳, 王为, 聂祚仁. 高等学校化学学报, 2010, 31(8), 1579-1584)
[21]	Hudson S., Cooney J., Hodnett B. K., Magner E., Chem. Mater.,2007, 19(8), 2049-2055
[22]	Dai J., Zou H., Wang R., Wang Y., Shi Z., Qiu S., Green Chem.,2017, 19(5), 1336-1344
[23]	Wang L., Chen C., Tian M. J., Yang Y. X., Luo J. H., Ding B., Wang P. M., J. Inorg. Mater.,2013, 29(4), 677-688
	(王磊, 陈诚, 田美娟, 杨宇翔, 罗健辉, 丁斌, 王平美. 无机材料学报, 2013, 29#(4), 677-688)
[24]	Wang M. M., Li Q. Y., Wei Q., Nie Z. R., Chem. J. Chinese Universities,2013, 34(2), 299-305
	(王苗苗, 李群艳, 韦奇, 聂祚仁. 高等学校化学学报, 2013, 34#(2), 299-305)
[25]	Zimmerman A. R., Goyne K. W., Chorover J., Komarneni S., Brantley S. L., Org. Geochem., 2004, 35(3), 355-375
[26]	Huan W., Yang Y., Wu B., Yuan H., Zhang Y., Liu X., Chinese J. Chem., 2012, 30(12), 2849-2860

Preparation of Yolk-shell Fe₃O₄@SiO₂@PMO Magnetic Microspheres for Laccase Immobilization^†

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 26

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHOU Ning, TANG Xiaohua, CAO Hong, ZHA Fei, LI Chun, XIE Chunyan, XU Mingping, SUN Yige. Preparation， Characterization and Degradation to BPA of Pomegranate-like Gel Microsphere Entrapmented Laccase [J]. Chem. J. Chinese Universities, 2022, 43(2): 20210705.
[2]	ZHU Haotian, JIN Meixiu, TANG Wensi, SU Fang, LI Yangguang. Properties of Transition Metal-biimidazole-Dawson-type Tungstophosphate Hybrid Compounds as Supports for Enzyme Immobilization [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220328.
[3]	WU Rong, DONG Qihui, SUN Yiyi, SU Erzheng. Efficient Enzyme Immobilization by Combining Adsorption and Cellulose Membrane Coating ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1888.
[4]	HUANG Xiaolin,XIE Huan,CAO Hong,JIN Hongjie,LI Chun,WU Tinghua. Preparation, Characterization of Magnetic Core Gel Microspheres Loaded Ionic Liquids and Its Application in Cells Immobilization Technique^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 793.
[5]	Xiaoyu FAN,Ke WANG,Shiyong SUN,Biaobiao MA,Rui LÜ. Construction and Catalytic Performances of Fe-aminoclay Nanostructured Lipase ^† [J]. Chem. J. Chinese Universities, 2019, 40(12): 2512.
[6]	GAO Fengqin,WANG Shan,WANG Yunfang,ZHAO Danlei,CUI Ru,JIANG Yucheng. Immobilization of Chloroperoxidase with Magnetic Graphene Oxide and Its Application of Decolorization of Acid Blue 45^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 904.
[7]	LI Wenjuan, ZHAO Yilei. Salt Tolerance of T. Versicolor Laccase: Bioinformatics Study and Internal Transportation of Chloride, Dioxygen, and Water^† [J]. Chem. J. Chinese Universities, 2018, 39(2): 255.
[8]	ZHOU Yamei, KONG Xiangzheng, HAN Hui, JIANG Xubao, ZHU Xiaoli. Easy Synthesis of Porous Polyurea and Its Application in Enzyme Immobilization and Kinetic Resolution of Racemic Phenylethanol^† [J]. Chem. J. Chinese Universities, 2017, 38(3): 495.
[9]	JIAO Jing, YANG Xue, JIN Lanna, GAO Jian, ZHOU Yang, XIAO Yazhong, ZHANG Yingjiu. Conservative and Variability of the Important Functional Sites in a Laccase from Bacillus Subtilis^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1320.
[10]	LI Chunyi, HUANG Zhuolie, WANG Chunxiao. Effects of Chemical Modification with Chitooligosaccharides on Catalytic Activity and Enzymological Properties of Laccase from Trametes versicolor^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 704.
[11]	XIA Ying, CAO Hao, ZHANG Yingjiu. Effect of Substrate Material on the Immobilization of Multifunctional Amylase^† [J]. Chem. J. Chinese Universities, 2015, 36(2): 330.
[12]	LI Qiujin, ZHAO Zhiqi, YUAN Yamei, GONG Jixian, ZHANG Jianfei. Immobilization of Papain with Ionic Liquids-regenerated Cellulose Membrane for Wool Fabric Modification^† [J]. Chem. J. Chinese Universities, 2015, 36(12): 2590.
[13]	SONG Min, ZHANG Linping, ZHONG Yi, XU Hong, MAO Zhiping. Catalytic Properties of Manganese Complex of Cyclic Polyamine Encapsulated in Ethyl Cellulose Microcapsules^† [J]. Chem. J. Chinese Universities, 2014, 35(9): 1941.
[14]	MU Yangyang, ZHEN Qiannan, WANG Mengfan, QI Wei, SU Rongxin, HE Zhimin. Preparation and Thermal Kinetic Deactivation of Cross-linked Enzyme Aggregates of Penicillin Acylase^† [J]. Chem. J. Chinese Universities, 2014, 35(6): 1212.
[15]	QI Yanbing, ZHU Jiren, SUN Yaojin, DU Yun, CHU Jianjun, SHI Ting, ZHAO Yilei, WANG Xiaolei. Theoretical Studies of the Binding-affinity and Reactivity Between Laccase and Phenolic Substrates^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 776.