Preparation of 20(S/R)-Ginsenoside Rg3 and Their Effects on Regulation of Th1/Th2 Imbalance†

doi:10.7503/cjcu20180372

Abstract

Abstract:

20(S)-Ginsenoside Rg₃ and 20(R)-ginsenoside Rg₃ were prepared by degradation and extraction methods from leaves of Panax quinquefolium L. The regulation effects of 20(S)-Rg₃ and 20(R)-Rg₃ on imbalance of Th1/Th2 cell were studied in vivo. As the orthogonal array optimization results shown, when the concentration of acetic acid was 50%, temperature of 80 ℃ and extraction time of 1 h, the conversion rates of 20(S)-Rg₃ and 20(R)-Rg₃ were highest at 12.30% and 14.80%, respectively. Then the 20(S/R)-Rg₃ were purified by chromatographic method. According to the experimental results of activity, 20(S/R)-Rg₃ inhibited the Th2 cells development by down-regulation of IL-4 production. It was associated with the CD40 and TIM-4 expression of LDCs. This work reports a new method to prepare ginsenoside Rg₃ and draw a novel biological mechanism of ginsenoside Rg₃, effects on imbalance of Th1/Th2.

Key words: Degradation, Extraction, 20(S/R)-Ginsenoside Rg₃, Th1/Th2 imbalance

CLC Number:

O629

TrendMD:

LIU Ying, CHEN Yanxin, WU Qian, LI Peng, LI Xuwen, SHI Xiaolei, JIN Yongri. Preparation of 20(S/R)-Ginsenoside Rg₃ and Their Effects on Regulation of Th1/Th2 Imbalance^†[J]. Chem. J. Chinese Universities, 2018, 39(11): 2419.

Figures/Tables 4

Fig.1 Formation of 20(S)-Rg3 and 20(R)-Rg3 in acetic acid extraction process

Fig.2 HPLC charomatograms of reference substance(A) and samples(B)

Fig.3 Effects of 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 on IFN-γ(A) and IL-4(B) in the culture supernatants of T lymph node cellsa. Control; b. 20(S)-Rg3; c. 20(R)-Rg3.Mean±SD, N=3; ** P<0.01 vs. control; ## P<0.01 vs. (S)-Rg3.

Fig.4 Effects of 20(S)-Rg3 and 20(R)-Rg3 on mRNA expression for surface molecules CD40, CD80, CD86 and TIM-4 on LDCsBands in (A) are quantified by desitometric analysis relative to β-actin and shown in (B). Mean±SD,N=3; * P<0.05 vs. control; ** P<0.01 vs. control; # P<0.05 vs. (S)-Rg3; ## P<0.01 vs. (S)-Rg3.

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Preparation of 20(S/R)-Ginsenoside Rg₃ and Their Effects on Regulation of Th1/Th2 Imbalance^†

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