Preparation, Characterization and Toxicological Analysis of Alginate-phospholipid Vesicle Composite Hydrogels†

doi:10.7503/cjcu20160919

Abstract

Abstract:

Phospholipid vesicles were synthesized by thin-film dispersion. According to the electric double layer theory of micelles, the vesicles were stabilized by electrolyte solutions, including manganese chloride, calcium chloride and magnesium chloride. There are no significant changes in the particle sizes of the vesicles colloid by the additions of manganese chloride and calcium chloride solutions, respectively. However, the particle sizes of the vesicles were significantly bigger by adding a certain amount of magnesium chloride. To further increase the stability of the phospholipid vesicles, alginate-phospholipid vesical composite hydrogels were prepared by dropping sodium alginate(SA) solution into phospholipid vesicle colloids stabilized by manganese chloride, calcium chloride and magnesium chloride, respectively. The results show that magnesium chloride and SA hardly formed hydrogel, and calcium chloride and SA formed hydrogel much easier than manganese chloride did. The phosphatidylserine(PS) in the phospholipid vesicles bound to the Ca²⁺ and Mg²⁺ through forming PS-Ca²⁺, PS-Mg²⁺, respectively, however PS cannot interact with Mn²⁺, forming PS-Mn²⁺. The morphology, swelling ratio and cytotoxicity of the composite hydrogels made of alginate-phospholipid vesicles were characterized. The results show that a novel composite hydrogel could be prepared by calcium-crosslinking SA and the PS in the phospholipid vesicles colloid, forming stable and non-cytotoxic composite hydrogel.

Key words: Phospholipid vesicle, Thermodynamic stability, Sodium alginate, Hydrogel, L-Phosphatidylserine

CLC Number:

TrendMD:

SHI Zhanping, SHI Mai, ZHANG Wenhui, SHEN Shigang, YUE Zhilian, YANG Hui, DING Liang, PAN Xuefeng. Preparation, Characterization and Toxicological Analysis of Alginate-phospholipid Vesicle Composite Hydrogels^†[J]. Chem. J. Chinese Universities, 2017, 38(7): 1270.

Figures/Tables 11

Fig.1 SEM image(A) and particle size distribution(B) of phospholipid microcapsules

Fig.2 Zeta potential distribution of phospholipid microcapsules colloids by adding electrolyte solutions (A) NaCl and KCl solutions; (B) MnCl2, CaCl2 and MgCl2 solutions.

Table 1 Diameter(D90) with different concentrations of NaCl/KCl added into the microcapsules colloids

Electrolyte solution	Concentration/ (mmol·L^-1)	Diameter(D90)/ nm	Electrolyte solution	Concentration/ (mmol·L^-1)	Diameter(D90)/ nm
NaCl	0	220.3±14.7	KCl	0	220.3±14.7
	12.50	217.0±4.1		12.50	224.9±10.2
	25.00	185.1±18.4		25.00	191.4±12.0
	50.00	180.5±15.4		50.00	202.3±6.2
	100.00	213.1±5.8		100.00	207.7±7.4
	150.00	223.1±3.9		150.00	218.7±9.1

Table 2 Diameter(D90) with different concentrations of MnCl2, CaCl2 and MgCl2 in the microcapsule colloids

MnCl₂ Concentration/ (mmol·L^-1)	Diameter(D90)/ nm	CaCl₂ Concentration/ (mmol·L^-1)	Diameter(D90)/ nm	MgCl₂ Concentration/ (mmol·L^-1)	Diameter(D90)/ nm
0	220.3±14.7	0	220.3±14.7	0	220.3±14.7
1.00	238.8±25.0	3.00	201.1±1.2	6.00	203.3±4.7
3.00	229.4±5.0	6.00	211.8±1.2	12.50	275.4±12.3
4.00	253.6±3.3	12.50	248.2±15.1	20.00	264.9±14.9
5.00	264.2±0.3	15.00	242.0±5.5	25.00	1019.2±73.9
6.00	208.6±10.3	18.00	243.3±9.0	26.00	874.0±49.6
9.00	221.3±7.6	20.00	223.2±4.2	27.00	761.4±17.4
12.50	238.5±0.2	22.00	217.3±3.5	28.00	810.4±87.1
25.00	225.3±1.1	25.00	235.7±3.6	29.00	771.8±53.8
35.00	212.2±20.9	35.00	205.6±5.94	35.00	466.4±28.2
45.00	223.6±0.8	45.00	219.3±11.1	45.00	214.6±15.8
50.00	253.9±2.0	50.00	210.1±0.8	50.00	224.7±10.4

Fig.3 Distributions of zeta potentials by adding sodium alginate into different colloidal dispersion systems

Scheme 1 Formation of alginate phospholpid microcapsules hydrogel

Scheme 2 Schematic illustrations of PS-Ca2+-SA crosslinking

Fig.4 SEM images of hydrogels(A) 12.50 mmol/L CaCl2 microcapsules colloidal+0.20%SA; (B) CaCl2+SA; (C) 6.00 mmol/L MnCl2 microcapsules colloidal+0.10%SA; (D) MnCl2+SA.

Fig.5 SEM images of colloidal microcapsules stabilized using CaCl2(A), SA+CaCl2 microcapsules colloidal(B) and CaCl2 solution form hydrogels(C)

Fig.6 Comparisons of swelling ratios of different hydrogelsa. Dropping SA(0.20%) into microcapsules colloids stabilized by CaCl2; b. CaCl2 solution alone; c. dropping SA(0.10%) into microcapsules colloid stabilized by MnCl2; d. MnCl2 solution alone.

Fig.7 Effects of composite hydrogels on cell proliferations of myocardial cells(A) and endothelial cells(B) a. Pure cells; b. extract solution; c. sodium alginate; d. phospholipid vesicles; e. phenol.

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