Rheological and Structural Characterization of Pectin Polysaccharides from Citrus Pulp†

doi:10.7503/cjcu20160066

Abstract

Abstract:

Raw pectin from citrus pulp was prepared with diluted acid extraction and ethanol precipitation. Its rheological behavior was assessed under different thermal treatments at different concentrations. Three polysaccharides, named P-0, P-1 and P-2, were isolated by anion-exchange chromatography on a diethyl-aminoethyl(DEAE) cellulose-52 column at 0, 0.1, 0.3 mol/L NaCl eluent, respectively. Their structural features were elucidated by determination of their molecular weight, sugar analyses, esterification degree analyses, Fourier transform infrared(FTIR) and ¹H nuclear magnetic resonance(¹H NMR) spectra. The results indicated that citrus pulp pectin was the typical shearing thinning non-newtonian fluid. The molecular weights of P-0, P-1 and P-2 were 752410, 286320, 131380, respectively. They belonged to the kind of acid polycose, which was abundant in uronic acid and low-esterified pectin with partial acetylesterification. P-0, P-1 and P-2 were mainly made up of rhammogalacturonan-Ⅰ(RG-Ⅰ) structure and their monosaccharides compositions were mostly glucuronate, galactose, arabinose, rhamnose and fucose at different ratio. FTIR and NMR spectra implied that P-0, P-1 and P-2 owned pyranose rings and exhibited both α- and β-glycosidic bonds.

Key words: Citrus pulp, Pectin, Structural feature, Polysaccharide, Rheological property

CLC Number:

O631
O629

TrendMD:

ZHI Zijian, ZOU Mingming, LI Shan, CHEN Jianle, YE Xingqian, CHEN Shiguo. Rheological and Structural Characterization of Pectin Polysaccharides from Citrus Pulp^†[J]. Chem. J. Chinese Universities, 2016, 37(6): 1175.

Figures/Tables 8

References 18

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Concentration of pectin solution(%)	Power-exponent equation	K/(Pa·sⁿ)	n
0.25	η=0.0119×γ^0.8766-1	0.0119	0.8766
0.50	η=0.0210×γ^0.8652-1	0.021	0.8652
0.75	η=0.0450×γ^0.8573-1	0.045	0.8573
1.00	η=0.0699×γ^0.8112-1	0.0699	0.8112

Concentration of pectin solution(%)	Power-exponent equation	K/(Pa·sⁿ)	n
0.25	η=0.0119×γ^0.8766-1	0.0119	0.8766
0.50	η=0.0210×γ^0.8652-1	0.021	0.8652
0.75	η=0.0450×γ^0.8573-1	0.045	0.8573
1.00	η=0.0699×γ^0.8112-1	0.0699	0.8112

Temperature/℃	Power-exponent equation	K/(Pa·sⁿ)	n
25	η=122.84×γ^0.7573-1	122.84	0.7573
40	η=66.78×γ^0.8079-1	66.78	0.8079
60	η=55.05×γ^0.8109-1	55.05	0.8109
80	η=53.48×γ^0.7157-1	53.48	0.7157
100	η=4.26×γ^0.8969-1	4.26	0.8969

Temperature/℃	Power-exponent equation	K/(Pa·sⁿ)	n
25	η=122.84×γ^0.7573-1	122.84	0.7573
40	η=66.78×γ^0.8079-1	66.78	0.8079
60	η=55.05×γ^0.8109-1	55.05	0.8109
80	η=53.48×γ^0.7157-1	53.48	0.7157
100	η=4.26×γ^0.8969-1	4.26	0.8969

Sample	Molar ratio(%)									10^-3 M_n	10^-3 M_w	D(M_w/M_n)
Sample	Man				Rha	GluA	GalA	Glu	Gal	Xyl	Ara	Fuc
P-0	0.68	8.22	0.81	35.50	3.27	15.80	6.09	17.81	11.83	201.72	752.41	3.73
P-1		8.53	0.93	42.37	1.55	10.67	4.45	11.72	19.78	86.24	286.32	3.32
P-2		11.12	0.59	42.88	1.46	6.43	3.50	8.98	25.04	75.07	131.38	1.75