Resonance Rayleigh Scattering Spectral Method for Determination of Methylene Blue with Gold Nanoparticle as Probe

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (5): 849.doi:

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Resonance Rayleigh Scattering Spectral Method for Determination of Methylene Blue with Gold Nanoparticle as Probe

LU Qun-Min, HE You-Qiu, LIU Shao-Pu*, LIU Zhong-Fang

School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)

Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
Contact: Shao-Pu

Abstract

Abstract:

In a neutral or weak alkaline medium(pH=6.5-9.5), the gold nanoparticle can combine with methylene blue by the virtue of electrostatic and hydrophobic interaction, forming aggregate with bigger diameters(average diameter increase from 12 to 20 nm ). The aggregate arouse Resonance Rayleigh Scattering(RRS) intensity enhancing greatly. The maximum scattering peaks are at 371 nm. Under optimum conditions, there is a linear relationship between scattering intensity(ΔI)　and the concentration of methylene blue. This method has a high sensitivity. So gold nanoparticle can be used as the RRS probe of high sensitivity for methylene blue. The detection limit of methylene blue is 21.17 ng/mL. The method which is simple and rapid has good selectivity. It was successfully applied to the detection of methylene blue in clinic serum samples.

Key words: Gold nanoparticle; Resonance Rayleigh scattering spectrum; Methylene blue

CLC Number:

O657

TrendMD:

LU Qun-Min, HE You-Qiu, LIU Shao-Pu*, LIU Zhong-Fang . Resonance Rayleigh Scattering Spectral Method for Determination of Methylene Blue with Gold Nanoparticle as Probe[J]. Chem. J. Chinese Universities, 2006, 27(5): 849.

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Resonance Rayleigh Scattering Spectral Method for Determination of Methylene Blue with Gold Nanoparticle as Probe

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