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Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (5): 845.doi:

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A New Method for Preparation of Gene Carriers Based on Silica Micro-particles

LIN Xia, HE Xiao-Xiao, WANG Ke-Min*, TAN Wei-Hong   

  1. State Key Laboratory of Chemo-Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
  • Contact: WANG Ke-Min

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Abstract:

A new method for the preparation of gene carriers based on silica micro-particles(SiMPs) was reported. Silica micro-particles were synthesized by the hydrolysis of tetraethoxy\|silane in ethanol and ammonia, and then modified with poly-L-lysine(PLL) molecules via the electrostatic interaction to form PLL-SiMPs complexes as the gene carriers. These gene carriers can deliver pEGFP into COS-7 cells to accomplish GFP expression. This new method is simple and rapid and will be of great potential to be widely applied in gene transfection and gene therapy.

Key words: Silica micro-particle; Gene carrier; Poly-L-lysine

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