Multi-walled Carbon Nanotubes Exposure Induces Oxidative Stress and Depolarizes Mitochondrial Membrane Potential in Cultured A549 Cells

Chem. J. Chinese Universities

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Multi-walled Carbon Nanotubes Exposure Induces Oxidative Stress and Depolarizes Mitochondrial Membrane Potential in Cultured A549 Cells

YE She-Fang^1*, ZHONG Li-Ming¹, WU Yi-Hui¹, ZHANG Qi-Qing^1,2*

1. Research Center of Biomedical Engineering﹠Department of Biomaterial, Research Center of Biomedical Engineering Technology of Xiamen City, The Key Laboratory of Biomedical Engineering of Fujian Province, College of Materials, Xiamen University, Xiamen 361005, China;
2. Institute of Biomedical Engineering, Chinese Academy of Medical Science﹠Peking Union Medical College, The Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China

Received:2008-06-24 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
Contact: YE She-Fang, ZHANG Qi-Qing

Abstract

Abstract: The present study was undertaken to determinate the cytotoxicity exhibited via multi-walled carbon nanotubes(MWCNTs) in human A549 lung epithelial cells as a model system. Cell mortality was measured by methyl thiazolyl tetrazolium(MTT) assays. The morphological changes of A549 cells were examined by Hoechst 33342 staining. The level of reactive oxygen species(ROS) was detected by means of flow cytometry analysis with a redox-sensitive fluorescent probe 2',7'-dichlorfluorescein-diacetate(DCFH-DA).The mitochondria membrane potential was observed by a confocal laser-scanning microscope with JC-1 fluorescence. The heme oxygenase-1(HO-1) protein expression was analyzed by immunofluorescence and Western blotting. The result reveal that treatment of A549 cells with MWCNTs lead to loss of cell viability, ROS production, glutathione depletion and decline in mitochondrial membrane potential. MWCNTs were potent toward inducing cellular heme oxygenase-1(HO-1) expression, a sensitive biomarker for oxidative stress. Our observations suggest that MWCNTs exert toxicity by increasing oxidative stress, decreasing the mitochondrial membrane potential in cultured A549 cells.

Key words: Multi-walled carbon nanotubes, Mitochondrial membrane potential, Oxidative stress, Heme oxygenase-1

CLC Number:

O629

TrendMD:

YE She-Fang^1*, ZHONG Li-Ming¹, WU Yi-Hui¹, ZHANG Qi-Qing^1,2*
. Multi-walled Carbon Nanotubes Exposure Induces Oxidative Stress and Depolarizes Mitochondrial Membrane Potential in Cultured A549 Cells
[J]. Chem. J. Chinese Universities, doi: .

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Multi-walled Carbon Nanotubes Exposure Induces Oxidative Stress and Depolarizes Mitochondrial Membrane Potential in Cultured A549 Cells

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