Capillary Electrophoresis Coupled with Tris(2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination

Abstract

Abstract:

The determination of bupivacaine by capillary electrophoresis with Ru(bpy)₃²⁺ electroche-miluminescence detection was described.The detection potential,Ru(bpy)₃²⁺ concentration,phosphate buffer concentration and pH,injection voltage and time were investigated.Experiment results showed that a good separation for the bupivacaine in plasma can be achieved under the optimized conditions,such as 10 s at 10 kV of electrokinetic injection,10 mmol/L separation phosphate buffer(pH=8.0),15 kV of separation voltage,5 mmol/L Ru(bpy)₃²⁺,and 50 mmol/L phosphate buffer(pH 6.5) in the ECL cell.The linear range was 0.02-10μg/mL,the relative standard derivation(RSD) was 3.4%,and the detection limit was 3 ng/mL.When the method was applied to measure bupivacaine in rat plasma,the results were satisfactory.

Key words: Bupivacaine, Capillary electrophoresis, Electrochemiluminesence

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WU Ya-Yan, LI Tian, XUE Jing, LIANG Heng. Capillary Electrophoresis Coupled with Tris(2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination[J]. Chem. J. Chinese Universities, 2004, 25(S1): 128.

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Capillary Electrophoresis Coupled with Tris(2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination

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