Preparation and Antioxidant Activities of Walnut Protein Hydrolysates

doi:10.7503/cjcu20120277

Abstract

Abstract:

Many studies have reported that peptides from various food sources possess antioxidant activity. In the present study, walnut proteins were hydrolyzed using Alcalase 2.4 L to obtain antioxidant peptides. The antioxidant activities of the hydrolysates were measured using 1,1-diphenyl-2-picryl hydrazyl(DPPH) assay and hydroxyl radical scavenging activity(HRSA) assay. Then, walnut protein hydrolysates were purified sequentially by gel filtration and RP-HPLC. The sequence of the peptide with the highest antioxidative activity was identified to be Ala-Gly-Gly-Ala using RP-HPLC-ESI-MS, which was identified for the first time from walnut protein hydrolysates. The reducing power of the peptide was similar to that of L-glutathione reduced(GSH). These results suggest that the peptide isolated from walnut protein hydrolysates is potent antioxidant and may be effectively used as food additives and as pharmaceutical agents.

Key words: Walnut protein hydrolysis, Antioxidant activity, Purification, Antioxidant peptide

CLC Number:

O657

TrendMD:

CHEN Ning, SUN Yi, LIU Shu-Ying. Preparation and Antioxidant Activities of Walnut Protein Hydrolysates[J]. Chem. J. Chinese Universities, 2013, 34(1): 72.

[1]	HUANG Luoyi, WENG Yueyue, HUANG Xuhui, WANG Chaojie. Theoretical Study on the Structures and Properties of Flavonoids in Plantain [J]. Chem. J. Chinese Universities, 2021, 42(9): 2752.
[2]	HU Haocheng, LI Wenli, ZHANG Jianing, LIU Yubo. Extraction， Structure Characterization and Biological Activities of Oligosaccharides from Auricularia heimuer [J]. Chem. J. Chinese Universities, 2021, 42(8): 2465.
[3]	WANG Yanbing,ZHANG Hui,YANG Junshan,CHEN Bingpeng,SUN Jiaming. Isolation, Identification and Antioxidant Activity of Lignans in Jasminum lanceolarium^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1942.
[4]	CAO Yuhui, ZHANG Juanjuan, WANG Zaiyang, ZHAO Yuanhui. Separation and Identification of Oyster Peptide Modified by Plastein Reaction and Characterization of Peptide-zinc Complexes^† [J]. Chem. J. Chinese Universities, 2018, 39(3): 470.
[5]	LIAN Xiaowei, HUANG Jingjing, TAO Zhu, ZHOU Qingdi, ZHANG Qianjun, WEI Gang. Host-guest Interaction Between Cucurbit[8]uril and Pinocembrin and Its Influence on the Properties of Pinocembrin^† [J]. Chem. J. Chinese Universities, 2018, 39(2): 226.
[6]	HUANG Jingjing, XU Zhiling, LIAN Xiaowei, ZHANG Xiaodong, TAO Zhu, ZHOU Qingdi, ZHANG Qianjun, WEI Gang. Host-guest Interaction Between Cucurbit[8]uril and Baicalin and Its Effect on the Properties of Baicalin^† [J]. Chem. J. Chinese Universities, 2018, 39(11): 2425.
[7]	ZHANG Luyun, XU Qian, XIA Guangqing, CONG Li, WANG Yu, ZHANG Hui, ZHU Junyi, ZANG Hao. Synthesis and Biological Activity of Lipoic Acid Ester Derivatives^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2198.
[8]	YANG Xin, ZHANG Di, SONG Limin, XU Qian, XU Hui, LIU Ke. Spectroscopic Studies on Alkaline Autoxidation of Curcumin and Antioxidative Activities of the Product^† [J]. Chem. J. Chinese Universities, 2017, 38(9): 1549.
[9]	LI Mengjie, LIAN Xiaowei, XU Zhiling, XING Xuyang, ZHANG Jianxin, LI Rui, TAO Zhu, ZHANG Qianjun. Influence of Interaction with Cucurbit[7]uril on Solubility, Stability and Antioxidant Activity of 2'-Hydroxychalcone^† [J]. Chem. J. Chinese Universities, 2017, 38(3): 442.
[10]	QIU Linzi, WANG Guirong, PAN Yan, WANG Yiwen, LI Xinqing, DENG Linhong, Mark Bradley, ZHANG Rong. Polymer Coatings for Purification and Long-term Culture of Human Adipose-derived Stem Cells^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1897.
[11]	LIU Yang, YIN Lu, GONG Guiping, PENG Yifang, HUANG Linjuan, WANG Zhongfu. Structural Characterization, Antioxidant Activity and Immunomodulatory Activity of the Polysaccharide LRLP3 from Leaves of Lycium ruthenicum Murra^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 261.
[12]	LIU Lei, LI Ke, HAO Xia, WANG Guizhen, QIN Xuemei, DU Guanhua, ZHANG Xiang. Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^† [J]. Chem. J. Chinese Universities, 2016, 37(12): 2168.
[13]	XU Yan, TIAN Shasha, YU He, YANG Wenzhi, ZHU Huajie. Isolation and Identification of Two New Compounds from Aspergillus Aculeatus^† [J]. Chem. J. Chinese Universities, 2015, 36(6): 1107.
[14]	ZHU Jinming, SUN Zhuo, FU Yao, GUO Yi, XU Li. Labeling and Purification of Alcalase and Fluorescent Nanocrystals Synthesized in Water Phase^† [J]. Chem. J. Chinese Universities, 2015, 36(3): 511.
[15]	SONG Panshu, WANG Jun, REN Tongxiang, ZHOU Tao, LU Hai. Novel Purification Method for Isotopically Enriched Molybdenum and Determination of Its Mass Fractionation Effect^† [J]. Chem. J. Chinese Universities, 2015, 36(2): 248.

Preparation and Antioxidant Activities of Walnut Protein Hydrolysates

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments