Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe

doi:10.7503/cjcu20120687

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (3): 551.doi: 10.7503/cjcu20120687

• Analytical Chemistry • Previous Articles Next Articles

Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe

YANG Shao-Ming, ZHA Wen-Ling, LI Hong, SUN Qing, LIU Bin, ZHENG Long-Zhen

Department of Chemistry and Chemical Engineering, School of Basic Sciences, East China Jiaotong University, Nanchang 330013, China

Received:2012-07-20 Online:2013-03-10 Published:2013-02-18
Contact: YANG Shao-ming E-mail:yangsm@yahoo.cn

Abstract

Abstract:

A lable-free aptamer biosensor was fabricated with the electropolymerized poly(thionine) as a redox electrochemical probe and gold nanoparticles as the immobilization matrix for aptamer. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of the aptamer biosensor. The electrochemical behavior of the aptamer biosensor was studied by the cyclic voltammetry(CV) and differential pulse voltammetry(DPV). The biosensor shows a good linear range of 1.0 pg/mL—500 ng/mL for thrombin, R=0.998, with a detection limit of 0.38 pg/mL. The biosensor has simple preparation procedure, high sensitivity and strong anti-interference ability.

Key words: Poly(thionine), Gold nanoparticles, Aptamer, Thrombin, Biosensor

CLC Number:

O657.1

TrendMD:

YANG Shao-Ming, ZHA Wen-Ling, LI Hong, SUN Qing, LIU Bin, ZHENG Long-Zhen. Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe[J]. Chem. J. Chinese Universities, 2013, 34(3): 551.

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Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe

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