Abstract: Determination of bisphenol A(BPA) of trace level in water was performed by dispersive liquid-liquid microextraction(DLLME) and high performance liquid chromatography. The optimal conditions of 22.5 μL of extraction solvent(chlorobenzene), 0.5 mL of disperser solvent(acetone), 0 min of extraction time, 10% of ionic strength, 3.2 of pH value and 9 mL of water sample volume, were obtained by screening and optimization design. Under the optimum conditions, the linear range is 0.50—100 μg/L(R²=0.9941) and the limit of detection is 0.10 μg/L for determination of BPA. The recovery from 87.8% to 111.0%, RSD of 8.3%(n=5) and enrichment factor from 1905 to 2527 were obtained at a mass concentration of BPA of 1 μg/L. The relative recoveries of BPA from tap water, river water and recycled water at spiking different levels are (108±11.1)%, (107±13.2)%, and (81.2±6.2)%(n=3), respectively. Moreover, under the determined chromatographic conditions, the effects of acetylene estradiol, estradiol, estriol, estrone and nonylphenol on the determination of BPA in water by high performance liquid chromatography with dispersive liquid-liquid microextraction were not of existence.

Key words: Dispersive liquid-liquid microextraction, High performance liquid chromatography, Bisphenol A, Chlorobenzene, Acetone, Estrogen