Abstract: 2-Methacryloyloxy ethyl phosphorylcholine(MPC) was prepared by the reaction of phosphorylcholine dichloride with 2-hydroxyethyl methacrylate(HEMA). The novel phosphocholine chromatographic stationary phase was synthesized by free radical graft polymerization of MPC from the surface of porous silica particles. The polymerization was initiated by thermal cleavage of tert-butylperoxy groups covalently attached to the particle surface. The stationary phase was evaluated in detail to determine its separability and to study the effect of pH on the retention of proteins. The dynamic protein loading capacity of the synthesized phosphocholine chromatographic stationary phase for Lys and BSA was 13.8 and 18.7 mg/g, respectively. The experimental results show that two acid proteins and two basic proteins were separated with 10 min linear gradient elution via the synthesized phosphocholine stationary phase.

Key words: Preparation of phosphocholine chromatographic stationary phases, Zwitterionic ion exchange packing, Separation of proteins