Detection of Cysteine in Aqueous Solution Based on Indicator Displacement by Ultraviolet-Visible and Colorimetric Methods

doi:10.3969/j.issn.0251-0790.2012.06.007

Abstract

Abstract: Through ultraviolet-visible(UV-Vis) spectra, a strategy for the determination of cysteine(Cys) was established by simply assembling copper(Ⅱ) and 4-(2-pyridylazo)-resorcinol(PAR) in a molar ratio of 1: 1 in aqueous solution. Cys could be detected in 10 mmol/L 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES) buffer(pH=7.0) system containing copper(Ⅱ)-PAR due to coordination competition. The Cu²⁺-PAR ensemble was highly selective for Cys without interference from other amino acids. From the UV-Vis absorbance value changes of Cu²⁺-PAR and Cu²⁺-PAR-Cys, the optimal coordinated ratio of PAR: Cu²⁺ was found to be 1: 1 and Cu²⁺:Cys was 1: 3. It is worthy mentioned that within the concentration range of 0—240 μmol/L in aqueous solution, the measurement of Cys conformed well with linear law, with an average coefficient of light extinction of 7519 L/(mol·cm) and a correlation coefficient of 0.9982. The whole recognition process for Cys gave rise to a rapid visual color change from pink to yellow which can be observed simulta-neously with naked eye.

Key words: Indicator displacement method, Copper(Ⅱ), 4-(2-Pyridylazo)-resorcinol, Cysteine, UV-Vis spectrum

CLC Number:

O657.32

TrendMD:

LI Yong-Ling, ZHANG Jian-Gang, WEI Yan-Li, CHENG Fang-Qin. Detection of Cysteine in Aqueous Solution Based on Indicator Displacement by Ultraviolet-Visible and Colorimetric Methods[J]. Chem. J. Chinese Universities, 2012, 33(06): 1158.

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