Chem. J. Chinese Universities ›› 2018, Vol. 39 ›› Issue (8): 1647.doi: 10.7503/cjcu20170806
• Analytical Chemistry • Previous Articles Next Articles
XIA Yuting, JIANG Bo, WU Qing, HU Qinghong, YUAN Zeli*()
Received:
2017-12-11
Online:
2018-08-10
Published:
2018-06-04
Contact:
YUAN Zeli
E-mail:zlyuan2002@126.com
Supported by:
CLC Number:
TrendMD:
XIA Yuting, JIANG Bo, WU Qing, HU Qinghong, YUAN Zeli. Synthesis and Application of Colorimetric Probe for the Simultaneous Detection of Sulphion and Homocysteine/Cysteine in the Serum Samples†[J]. Chem. J. Chinese Universities, 2018, 39(8): 1647.
Fig.1 Absorbance spectra change of probe 1(20 μmol/L) in Tris-HCl(10 mmol/L, pH=7.4, 50% ethanol) upon individual addition of 100 μmol/L different anions(A) and fluorescence spectra change of probe 1(20 μmol/L) in Tris-HCl(10 mmol/L, pH=7.4, 50% ethanol) upon individual addition of 100 μmol/L different amino acids(B)Inset: color changes of probe 1 solution before(left) and after(right) the addition of S2-(A) and Cys(B). (A) Other anions include F-, Cl-, Br-, I-, NO3-, CN-, PO43-, HPO42-, H2PO4-, SCN-, SO42-, HSO4-, CO32-, HCO3-, ClO3-, IO4-, CH3COO-; (B) other amino acids include glycine, serine, aspartic acid, glutathione, tyrosine, glutamic acid, arginine, tryptophan, valine, histidine, methionine, phenylalanine, phenylglycine, alanine, leucine, methionine.
Fig.2 Absorbance spectra of probe 1(20 μmol/L) in the presence of S2-(0—42 μmol/L)(A) and luorescece spectra of probe 1(20 μmol/L) in the presence of Hcy(0—100 μmol/L)(B)
Fig.3 Absorbance(552 nm) changes of probe 1(20 μmol/L) in Tris-HCl(10 mmol/L, pH=7.40, 50% ethanol) solution upon individual addition of 44 μmol/L miscellaneous anions and followed by further addition of 44 μmol/L S2-(A) and fluorescence intensity(552 nm) changes of probe 1(20 μmol/L) in Tris-HCl(10 mmol/L, pH=7.40, 50% ethanol) solution upon individual addition of 100 μmol/L amino acids and followed by further addition of 100 μmol/L of Hcy(A) a. S2-; b. S2-+F-; c. S2++SCN-; d. S2-+Cl-; e. S2-+SO42-; f. S2-+Br-; g. S2-+I-; h. S2-+HSO3-; i. S2-+NO3-; j. S2-+CN-; k. S2-+CO32-; l. S2-+PO43-; m. S2-+HCO3-; n. S2-+HPO42-; o. S2-+ClO3-; p. S2-+IO4-; q. S2-+H2PO4-; r. S2-+Ac-. (B) a. Hcy; b. Hcy+GSH; c. Hcy+Try; d. Hcy+Glu; e. Hcy+Arg; f. Hcy+Gly; g. Hcy+Trp; h. Hcy+Asn; i. Hcy+Val; j. Hcy+Hcy; k. Hcy+His; l. Hcy+Met; m. Hcy+Ser; n. Hcy+Phe; o. Hcy+Phg; p. Hcy+Cys; q. Hcy+Ala; r. Hcy+Leu; s. Hcy+Mts.
Fig.4 Partial 1H NMR spectra of probe 1(a), product of probe 1 with 0.835 mol/L(b) and 1.67 mol/L(c) S2- in DMSO-d6/D2O(volume ratio 9:1) and the intermidate(d)
Fig.6 Color changes of the filter paper coated with probe 1(20 μmol/L) used for the detection of S2-(A) and Cys(B)(A) a. Only probe 1; b. 1+F-; c. 1+Cl-.; d. 1+Br-; e. 1+ I-; f. 1+NO3-; g. 1+S2-; h. 1+SO42-; i. 1+HSO4-; j. 1+CO32-; k.1+HCO3-; l. 1+ClO32-; m. 1+PO43-; n. 1+H2PO4-; o. 1+HPO42-; p. 1+SCN-; q. 1+IO4-; r. 1+CN-; s. 1+AcO-. (B) a. Only probe 1; b. 1+Hcy; c. 1+Met; d. 1+Arg; e. 1+Gly; f. 1+Trp; g. 1+GSH; h. 1+Ser; i. 1+His; j. 1+Lys; k. 1+Val; l. 1+Phg; m. 1+Phe; n. 1+Ala; o. 1+cys; p. 1+Tyr; q. 1+Asn; r. 1+Leu; s. 1+Glu.
Fig.7 Color changes of the filter paper coated with probe 1(20 μmol/L) used for the detection of S2-(A) and Cys(B)(A)c(S2-)/(μmol·L-1): a. 0; b. 6.0; c. 12.0; d. 18.0; e. 24.0; f. 30.0; g. 40.0. (B) c(Cys)/(μmol·L-1): a. 0; b. 10.0; c. 30.0; d. 40.0; e. 50.0; f. 60.0; g. 80.0.
Analyte | c(Spiked)/(μmol·L-1) | Quantities measured/(μmol·L-1) | Average recovery(%) | RSD(%)(n=5) |
---|---|---|---|---|
4 | 3.9 | 96.4 | 2.0 | |
6 | 6.3 | 105.8 | 7.6 | |
8 | 7.6 | 95.3 | 2.2 | |
Cysb | 14 | 13.8 | 98.2 | 2.6 |
16 | 15.9 | 99.5 | 3.6 | |
18 | 17.7 | 98.3 | 2.3 | |
Hcyb | 16 | 15.9 | 99.3 | 5.3 |
18 | 17.9 | 99.4 | 4.9 | |
20 | 19.4 | 97.2 | 6.7 |
Table 1 Determination of S2-, Hcy and Cys in the serum samples
Analyte | c(Spiked)/(μmol·L-1) | Quantities measured/(μmol·L-1) | Average recovery(%) | RSD(%)(n=5) |
---|---|---|---|---|
4 | 3.9 | 96.4 | 2.0 | |
6 | 6.3 | 105.8 | 7.6 | |
8 | 7.6 | 95.3 | 2.2 | |
Cysb | 14 | 13.8 | 98.2 | 2.6 |
16 | 15.9 | 99.5 | 3.6 | |
18 | 17.7 | 98.3 | 2.3 | |
Hcyb | 16 | 15.9 | 99.3 | 5.3 |
18 | 17.9 | 99.4 | 4.9 | |
20 | 19.4 | 97.2 | 6.7 |
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