Chem. J. Chinese Universities

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Differential Chronopotentiometric Stripping Method for the Determination of Purines and DNA Concentration Using an Electrochemically Pretreated Glassy Carbon Electrode

JIA Wen-Li, LI Tian-Hua, ZHANG Jing-Jing, WANG Huai-Sheng
  

  1. Institute of Analytical Chemistry, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China
  • Received:2005-11-15 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10
  • Contact: WANG Huai-Sheng

Abstract: The differential chronopotentiometric stripping method wasused for the sensitive and selective determination of DNA and purines bases or the nucleosides concentrations in 0.1 mol/L phosphate buffer solution with pH=4.0 by using an electrochemically pretreated glassy carbon electrode. The results indicate that the electrochemically modified GCE could accumulate purines and ssDNA by effective adsorption and produce sensitive differential stripping peaks. The stripping peak heights(dt/dE) were linear with the concentrations of purine bases and denatured DNA in the suitable range. Some useful information related to DNA damage could be also acquired.

Key words: DNA, Purine bases and the corresponding nucleosides, Electrochemically pretreated glassy carbon electrode, Chronopotentiometric stripping analysis

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