The Complexing of Glucoamylase with Fluorescent Probe Tb(Ⅲ)

Abstract

Abstract: The bonding properties, enviroment and the dissociation constant of glucoamylase with Tb(Ⅲ) as a fluorescent probe were investigated. The results show that an enzyme molecule could be bonded with 4 Tb(Ⅲ) at 20℃> pH 4.6, and the bonding site is in the free carboxyl groups which belong to Glu/Asp, KD was calculated to be 2. 5×10^-4mol/L. The fluorescence intensity of Tb(Ⅲ) is greatly sensitized by the enzyme to 1000 folds at 545 nm. On the contrary, the fluorescence intensity of glucoamylase at 345 nm is quenched and the quenching efficiency is calculated to be 0. 75%.

Key words: Tb(Ⅲ), Fluorescence probe, Glucoamylase, Bonding property, Complexing

TrendMD:

Zhai Guo-tang, Yuan Jing-ming, Yuan Tao. The Complexing of Glucoamylase with Fluorescent Probe Tb(Ⅲ)[J]. Chem. J. Chinese Universities, 1991, 12(7): 939.

References

Related Articles 15

[1]	ZHENG Anni, JIN Lei, YANG Jiaqiang, WANG Zhaoyun, LI Weiqing, YANG Fangzu, ZHAN Dongping, TIAN Zhongqun. Effects of 5，5-Dimethylhydantoin on Electroless Copper Plating [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220191.
[2]	Yingying ZHANG,Yiwen HUANG,Bing ZHAO,Liyan WANG,Bo SONG. Synthesis of a Colorimetric Fluorescent Probe of Cr ³⁺ and Its Application in Cell Imaging ^† [J]. Chem. J. Chinese Universities, 2019, 40(12): 2486.
[3]	YU Zhaochuan, MA Wenhui, WU Tao, WEN Jing, ZHANG Yong, WANG Liyan, CHU Hongtao. Preparation of B, N, S co-Doped Graphene Quantum Dots for Fluorescence Detection of Fe ³⁺ and H₂P $O 4 - †$ [J]. Chem. J. Chinese Universities, 2019, 40(11): 2286.
[4]	HUANG Chibao,PAN Qi,CHEN Huashi,LIANG Xing,LÜ Guoling. Dicyanostilbene-derived Two-photon Fluorescence Probe for Lead Ions^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 897.
[5]	HAN Ruixia,LÜ Jitao,ZHANG Shuzhen. Molecular Probe for the Determination of Hydroxyl Radicals in Heterogeneous Systems: Coumarin^† [J]. Chem. J. Chinese Universities, 2018, 39(12): 2658.
[6]	XU Wanzhen, QIU Chunxiao, HUANG Weihong, LIU Hong, YANG Wenming. Computer Simulation Design, Preparation and Application of Fluorescence Sensors Based on Quantum Dots for Selective Detection of 4-Nitrophenol in River Water^† [J]. Chem. J. Chinese Universities, 2017, 38(7): 1155.
[7]	HUANG Chibao, PAN Qi, CHEN Xiaoyuan, ZHAO Guanglian, CHEN Huashi, LIANG Xing, LÜ Guoling. Dicyanostilbene-derived Two-photon Fluorescence Probe for Mercury Ions^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1751.
[8]	WANG Caiping, LI Youfen, ZHANG Yidong. Preparation of Glass Ceramics Containing CaF₂ by One-step Method and Analysis of the Fluorescent Probe of Eu³⁺ Ion^† [J]. Chem. J. Chinese Universities, 2016, 37(4): 607.
[9]	SONG Chunxia, YANG Xiaohai, WANG Kemin, WANG Qing, LIU Jianbo, HUANG Jin, LI Wenshan, HUANG Haihua, LIU Wei. Application of Polymers in Fluorescence Analysis^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 201.
[10]	MI Xiaolong, JIAO Xiaojie, LIU Chang, HE Song, ZENG Xianshun. Rhodamine-based Cell Permeable Fluoresecent Turn-on Probes for Cupric Ion^† [J]. Chem. J. Chinese Universities, 2016, 37(10): 1784.
[11]	HUANG Chibao, LIANG Xing, ZENG Qihua, CHEN Huashi, ZENG Boping, YI Daosheng, CHEN Xiaoyuan. Dicyanostilbene-derived Two-photon Fluorescence Probe for Free Zinc Ions in Live Cells and Living Tissues^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 646.
[12]	ZHOU Mingsong, WANG Wenli, YANG Dongjie, QIU Xueqing. Adsorption Driving Force of Sodium Lignosulphonate on Fe2O3 Surface [J]. Chem. J. Chinese Universities, 2014, 35(8): 1828.
[13]	ZHOU Mingsong, WANG Wenli, YANG Dongjie, QIU Xueqing. Adsorption Driving Force of Sodium Lignosulphonate on Fe₂O₃ Surface^† [J]. Chem. J. Chinese Universities, 2014, 35(8): 1822.
[14]	XU Hui, DAI Yanna, SHAN Hongyan, FEI Qiang, HUAN Yanfu, LI Guanghua, FENG Guodong. Rhodamine Derivative ABDO/Er³⁺ Composite Fluorescent Probe in Response to Temperature^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 736.
[15]	CHEN Yi, ZHANG Yan, ZENG Xi, MU Lan, LI Jun, SUN Qiang, ZHANG Jian-Xin, WEI Gang. Synthesis and Probe Properties of Rhodamine-triazine Derivatives [J]. Chem. J. Chinese Universities, 2013, 34(7): 1598.