Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (9): 1678.doi: 10.7503/cjcu20160277
• Physical Chemistry • Previous Articles Next Articles
HUO Yilin1, SHI Ying1, WANG Qinyue1, LI Luoyuan1, YU Longjiang2,3, WANG Peng1,*(), ZHANG Jianping1, WANG Zhengyu2
Received:
2016-04-25
Online:
2016-09-10
Published:
2016-08-26
Contact:
WANG Peng
E-mail:wpeng@iccas.ac.cn
Supported by:
CLC Number:
TrendMD:
HUO Yilin, SHI Ying, WANG Qinyue, LI Luoyuan, YU Longjiang, WANG Peng, ZHANG Jianping, WANG Zhengyu. Spectroscopic Properties of LH2 from Thermochromatium tepidum in Liposome and Detergent Micelles†[J]. Chem. J. Chinese Universities, 2016, 37(9): 1678.
Fig.1 Absorption of LH2 from Tch. tepidum in various environments at pH=8 a. in DMM; b. in LDAO; c. in liposome. The arrow shows the absorption of spirilloxanthin.
Fig.3 DLS data of the LH2 from Tch. tepidum in various detergents micelles or liposome systems a. in DMM(106 nm/565 nm); b. in LDAO(14 nm/257 nm); c. in liposome(73 nm). The data in the corresponding parentheses are the Rh of the vesicles for each system.
Fig.5 Qy absorption comparison of LH2 from Tch. tepidum in 0.05% DDM(a), 0.1% LDAO(b) and PC(c) liposome, under pH=8(A, C) and 3(B, D), and without Ca2+(A, B) or with 200 mmol/L Ca2+(C, D)(all the spectra are normalized at 797 nm), respectively Arrows in (A) show the splitting peaks position of B800.
System | Without Ca2+ | With 200 mmol/L Ca2+ | ||||||
---|---|---|---|---|---|---|---|---|
pH=8 | pH=3 | pH=8 | pH=3 | |||||
λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | |
In DDM | 855 | 1.41 | 854 | 1.28 | 857 | 1.53 | 855 | 1.35 |
In LDAO | 845 | 0.96 | 845 | 0.90 | 854 | 1.32 | 843 | 1.02 |
In liposome | 850 | 1.05 | 848 | 1.01 | 854 | 1.33 | 846 | 1.26 |
In chromatophoreb | 854 | 1.41 | 852 | 1.18 | 858 | 1.38 | 857 | 1.32 |
Table 1 Qy absorption characteristics of LH2 from Tch. tepidum under various conditionsa
System | Without Ca2+ | With 200 mmol/L Ca2+ | ||||||
---|---|---|---|---|---|---|---|---|
pH=8 | pH=3 | pH=8 | pH=3 | |||||
λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | λmax, B850/nm | ODB850/ODB800 | |
In DDM | 855 | 1.41 | 854 | 1.28 | 857 | 1.53 | 855 | 1.35 |
In LDAO | 845 | 0.96 | 845 | 0.90 | 854 | 1.32 | 843 | 1.02 |
In liposome | 850 | 1.05 | 848 | 1.01 | 854 | 1.33 | 846 | 1.26 |
In chromatophoreb | 854 | 1.41 | 852 | 1.18 | 858 | 1.38 | 857 | 1.32 |
Fig.6 Molecular structure and components of detergents(A, B) or lipid(C), molecular structure and components of lipids of chromatophore from Tch. Tepidum(D) and the amino acid sequence of α-apoproteins of LH2 from Tch. Tepidum[4](E) R1—R4 are the aliphatic chain with various length and degree of unsaturation. The gray broken line indicates the B850 bacteriochlorophyll binding histidine, and the red broken line indicates the possible binding points for calcium ion or proton.
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