Preparation and Properties of High-Density HBsAg-Conjugated Magnetic Probes

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (5): 1070.

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Preparation and Properties of High-Density HBsAg-Conjugated Magnetic Probes

ZHANG Bao-Liang, ZHANG Qiu-Yu*, ZHANG He-Peng, LIU Yu, FAN Xin-Long, XU Hai-Long

Department of Chemistry, College of Science, Northwestern Polytechnical University, Xi′an 710129, China

Received:2010-05-28 Revised:2010-08-09 Online:2011-05-10 Published:2011-04-11
Contact: ZHANG QiuYu E-mail:qyzhang2008@126.com
Supported by:
国家自然科学基金(批准号: 50773063)、国家“九七三”计划项目(批准号: 2010CB635111)、西北工业大学基础研究基金(批准号: G9KY1020)和西北工业大学研究生创业种子基金(批准号: Z2011009)资助.

Abstract

Abstract: The preparation of magnetic beads loaded with high-density Hepatitis B Surface Antigen (HBsAg) detection probes was introduced in this paper, and the properties of the beads for HBsAg detection were also studied. The immunomagnetic beads coupled with HBsAb were firstly prepared by chemical bond. Then, immunomagnetic beads were used for detecting HBsAg and were obtained a favorable effect. In order to improve the accuracy and sensitivity of detection, the preparation process of immunomagnetic beads was optimized, including amination and coupling process. Through the optimization, the amino content of magnetic beads was 2.71mmol/g, unit magnetic beads bonded 108.36 ug/mg antibodies and the coupling efficiency was 77.40%. The activity of HBsAb on magnetic beads was detected by the method similar to Enzyme-Linked Immunosorbent Assay (ELISA). Compared with common ELISA technology, magnetic beads loaded with high-density HBsAb have higher sensitivity.

Key words: immunomagnetic beads, surface functionalization, HBsAg detection, amination

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ZHANG Bao-Liang, ZHANG Qiu-Yu*, ZHANG He-Peng, LIU Yu, FAN Xin-Long, XU Hai-Long. Preparation and Properties of High-Density HBsAg-Conjugated Magnetic Probes[J]. Chem. J. Chinese Universities, 2011, 32(5): 1070.

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Preparation and Properties of High-Density HBsAg-Conjugated Magnetic Probes

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