Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (4): 851.

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Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE

XIA Zhi-Ning^1,2*, LI Li-Xian^1,2, CHEN Hua², XIONG Cai-Qiao³, YANG Feng-Qing²

1. College of Bioengineering,
2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China;
3. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China

Received:2010-07-13 Revised:2010-08-23 Online:2011-04-10 Published:2011-03-09
Contact: XIA Zhi-Ning E-mail:chem_lab_cqu@yahoo.com.cn
Supported by:
国家自然科学基金(批准号: 20775096, 20805060)资助.

Abstract

Abstract: Based on the theory of plug-plug kinetic capillary electrophoresis (ppKCE), the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug for the first time. The rate constants of complex formation kon, the dissociation koff and the binding constant Kb between cell membrane and drugs were directly determined simultaneously. The stability of the peaks height and the migration times were studied, and the influence of pH on the kinetic parameters of the interaction between the cell membrane and drugs were examined and analyzed. It could provide a new approach for the evaluation of drug efficiency, toxicity and pharmacokinetics in vivo.

Key words: Plug-plug kinetic capillary electrophoresis(ppKCE), Cell membrane, Kinetic parameter, Interaction

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XIA Zhi-Ning*, LI Li-Xian, CHEN Hua, XIONG Cai-Qiao, YANG Feng-Qing. Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE[J]. Chem. J. Chinese Universities, 2011, 32(4): 851.

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Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE

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