Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (12): 2891.

• Articles • Previous Articles     Next Articles

Effects of collagen liquid crystalline films on cell culture

TANG Min-Jian, DING Shan, MIN Xiang, JIAO Yan-Peng, ZHOU Chang-Ren*, LI Hong   

  1. Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510630, China
  • Received:2011-04-20 Revised:2011-05-24 Online:2011-12-10 Published:2011-11-25
  • Contact: ZHOU Chang-Ren E-mail:tcrz9@jnu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 31070852)和教育部博士学科点基金(批准号: 200805590001)资助.

Abstract: A series of biomimetic collagen films was prepared. The texture and topological structure of collagen films were studied by polarizing optical microscopy(POM), scanning electron microscopy(SEM) and atomic force microscopy(AFM). The relationship between collagen membranes and 3T3 cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) method and cytoskeleton fluorescence staining. The results show that the collagen liquid crystal(LC) membranes present continuously twisting orientations in cholesteric phases characterized by typical series of arced patterns. Compared with the non-LC membranes, LC membranes not only could promote the cell's adhesion and proliferation, but also the LC topological structure had a contact guide effect to 3T3 cells.

Key words: Collagen, Liquid crystal, Topological structure, Contact guide

CLC Number: 

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