Isolation and Structure Characterization of Polysaccharide from Atractylodes Macrocephala Koidz

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (12): 2812.

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Isolation and Structure Characterization of Polysaccharide from Atractylodes Macrocephala Koidz

WU Le-Qin¹, ZHANG Jing^1*, SUN Run-Guang², JIANG Shao-Fen¹, WANG Yan-Li¹, DAI Jing-Jing¹

1. College of Food Engineering and Nutrition Science,
2. Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shanxi Normal University, Xi'an 710062, China

Received:2010-12-27 Revised:2011-02-27 Online:2011-12-10 Published:2011-11-25
Contact: ZHANG Jing E-mail:zhangjin@snnu.edu.cn
Supported by:
国家自然科学基金(批准号: 10874108)和陕西省自然科学基础研究计划项目(批准号: SJ08A16)资助.

Abstract

Abstract: Crude polysaccharides, extracted from the stem of Atractylodes macrocephala Koidz by hot water(80 ℃), were fractionated by DEAE-52 anion exchange chromatography and purified by Sephadex G-200 gel filtration chromatography to afford a fraction, named WAM. The polysaccharide(WAM) was subjected to structural identification by a combination of chemical and instrumental analysis. The molecular weight of WAM was measured to be 3263 by high performance liquid chromatography. Gas chromatography analysis indicated that WAM composed of glucopyranose and galactopyranose in a molar ratio of 3.01:1.00. The infrared spectra and nuclear magnetic resonance(NMR) spectra further confirmed that the configuration of D-glucopyranose and D-galactopyranose were of β-form. On the basis of partial acid hydrolysis, periodate oxidation, Smith degradation and methylation analysis, it can be concluded WAM had a backbone consisting of (1→3)-linked-β-D-glucopyranosyl and (1→3,6)-linked-β-D-glucopyranosyl, which was branched with a glycosyl residue, β-D-galactopyanose(1→ linked residue, at the C6 position of glucosyl along the main chain.

Key words: Atractylodes macrocephala Koidz, Polysaccharide, Isolation, Purification, Structure characterization

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WU Le-Qin, ZHANG Jing*, SUN Run-Guang, JIANG Shao-Fen, WANG Yan-Li, DAI Jing-Jing. Isolation and Structure Characterization of Polysaccharide from Atractylodes Macrocephala Koidz[J]. Chem. J. Chinese Universities, 2011, 32(12): 2812.

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Isolation and Structure Characterization of Polysaccharide from Atractylodes Macrocephala Koidz

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