Aptamer-modified AuRu Nanoparticle Catalytic Resonance Scattering Spectral Assay for the Determination of Trace Pb2+

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (12): 2366.

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Aptamer-modified AuRu Nanoparticle Catalytic Resonance Scattering Spectral Assay for the Determination of Trace Pb²⁺

LIANG Ai-Hui, ZHANG Jing, WEN Gui-Qing, LIU Qing-Ye, LI Ting-Sheng, JIANG Zhi-Liang*

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource Sciences, Guangxi Normal University, Guilin 541004, China

Received:2010-05-06 Revised:2010-05-24 Online:2010-12-10 Published:2010-12-06
Contact: JIANG Zhi-Liang E-mail:zljiang@mailbox.gxnu.edu.cn
About author:蒋治良, 男, 博士, 教授, 博士生导师, 从事纳米材料化学与光谱分析研究. E-mail: zljiang@mailbox.gxnu.edu.cn
Supported by:
国家自然科学基金(批准号: 21075023, 20865002, 20965002)、广西省自然科学基金(批准号: 0991021z)和珍稀濒危动植物生态与环境保护省部共建教育部重点实验室项目资助.

Abstract

Abstract: The Au/Ru nanoparticle, in the mole ratio of 5/1, was modified by aptamer to prepare an aptamer AuRu nanoprobe (AptAuRu) for Pb2+, and the AptAuRu probe was stable in pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of 85 mmol/L NaCl. Upon addition of Pb2+, the nanoprobe reacted with Pb2+ to form very stable G-quadruplex, and the released AuRu nanoparticles aggregated to big particles, which result to the resonance scattering (RS) peak at 592 nm increasing. The increased RS intensity was linear to the concentration of Pb2+ in the range of 1.2-240 pmol L-1. After filtration, the unreacted AptAuRu nanoprobe in filtration solution exhibited strong catalytic effect on the slow particle rection of NaClO3-NaI-TDMAC, and the association complex particle appeared a resonance scattering peak at 472 nm. When the concentration of Pb2+ increased, the amount of AptAuRu nanoprobe in filtration solution decreased, and the catalysis weak, that result to the resonance scattering intensity at 472 nm decreasing. The decreased intensity ΔI472nm was linear to the Pb2+ concentration in the range of 0.12～60 pM, with a regression equation of ΔI472nm =3.1C + 7.3 and a detection limit of 0.08 pM Pb2+. The RS assay was applied to analysis of Pb2+ in waste water, with satisfactory results.

Key words: Pb2+, aptamer, AuRu nanoparticle, catalytic resonance scattering spectral assay

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LIANG Ai-Hui, ZHANG Jing, WEN Gui-Qing, LIU Qing-Ye, LI Ting-Sheng, JIANG Zhi-Liang*. Aptamer-modified AuRu Nanoparticle Catalytic Resonance Scattering Spectral Assay for the Determination of Trace Pb²⁺[J]. Chem. J. Chinese Universities, 2010, 31(12): 2366.

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Aptamer-modified AuRu Nanoparticle Catalytic Resonance Scattering Spectral Assay for the Determination of Trace Pb²⁺

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