Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (11): 2167.

• Articles • Previous Articles Next Articles

Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

YUAN Yuan1,2,4, HE Xiao-Xiao1,2,4*, SHI Hui2,3,4, WANG Ke-Min2,3,4*, WU Xu1,2,4, HUO Xi-Qin2,3,4

1. College of Biology,
2. State Key Laboratory of Chemo/Biosensing and Chemometrics,
3. College of Chemistry and Chemical Engineering,
4. Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China

Received:2010-04-16 Online:2010-11-10 Published:2010-11-10
Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn; HE Xiao-Xiao. E-mail: xiaoxiaohe@hnu.cn
Supported by:
国家自然科学基金(批准号: 90606003, 20775021)、教育部创新研究群体项目、教育部科学技术重点项目(批准号: 107084)、新世纪优秀人才支持计划(批准号: NCET-06-0697)和湖南省自然科学基金创新研究群体项目(批准号: 10JJ7002)资助.

Abstract

Abstract: Gold nanocluster was synthesized by bovine serum albumin directing, and was characterized by fluorescence spectrophotometer, zeta sizer and non-denaturing polyacrylamide gel electrophoresis. The results show that the gold nanocluster’s fluorescence signal is strong and stable at different pH value. The interaction between gold nanoclusters and HeLa cells was further studied. It demonstrates that the gold naoclusters could be successfully uptaken by living cells. The good effects of the fluorescent labeling of living cells can be achieved by optimal incubation time and gold nanoclusters’ concentration. Moreover, the fixation treatment did not change the form of labeling. These results provide some guidance to the further application of fluorescent gold nanoclusters in the field of live cells labeling.

Key words: Gold nanocluster, Living cell, Fluorescent labeling

TrendMD:

YUAN Yuan, HE Xiao-Xiao*, SHI Hui, WANG Ke-Min*, WU Xu, HUO Xi-Qin. Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters[J]. Chem. J. Chinese Universities, 2010, 31(11): 2167.

References

[1]Torvinen M., Gine S. S., Hillion J., Latinic S., Canalsb M., Ciruelab F., Bordonic F., Stainesa W., Pedatac F., Agnatid L. F., Lluisb C., Francob R., Ferrée S., Fuxe K.. Neuroscience[J], 2002, 113(3): 709—719 [2]Zaccolo M.. Circulation Research[J], 2004, 94: 866—873 [3]Cossarizza A., Ceccarelli D., Masini A. A.. Experimental Cell Research[J], 1996, 222: 84—94 [4]LIANG Chong-Yang(梁重阳), XU Wei-Qing(徐蔚青), CAO Yan-Xin(曹焱鑫), LIU Li-Xia(刘立侠), ZHANG Shu-Qin(张淑芹), LIU Zhi-Yi(刘志屹), LI Hong-Rui(李泓睿), LI Bai-Zhi(李柏志), SUN Fei(孙非). Chem. J. Chinese Universities(高等学校化学学报)[J], 2009, 30(3): 489—492 [5]Barat B., Sirk S. J., McCabe K. E., Li J., Lepin E. J., Remenyi R., Koh A. L., Olafsen T., Gambhir S. S., Weiss S., Wu A. M.. Bioconjugate Chem.[J], 2009, 20: 1474—1481 [6]Rajan S. S., Vu T. Q.. Nano Letters[J], 2006, 6(9): 2049—2059 [7]XU Hong(徐红), YANG Shu-Min(杨淑敏), CHEN Geng(陈更), TIAO Lei-Lei(田雷蕾), HE Feng(何凤), WANG Guang-Yi(王广义), MA Yu-Guang(马於光). Chem. J. Chinese Universities(高等学校化学学报)[J], 2010, 31(4): 632—634 [8]WANG Yang(王洋), DENG Yu-Lin(邓玉林), QING Hong(庆宏), XIE Hai-Yan(谢海燕). Chem. J. Chinese Universities(高等学校化学学报)[J], 2008, 29(4): 661—668 [9]Duan H. W., Nie S. M.. J. Am. Chem. Soc.[J], 2007, 129: 2412—2413 [10]Liu X. F., Li C. H., Xu J. L., Lü J., Zhu M., Guo Y. B., Cui S., Liu H. B., Wang S., Li Y. L.. J. Phys. Chem. C[J], 2008, 112: 10778—10783 [11]Zheng J., Petty J. T., Dickson R. M.. J. Am. Chem. Soc.[J], 2003, 125: 7780—7781 [12]Yu J. H., Choi S., Richards C. I., Antoku Y., Dickson R. M.. Photochemistry and Photobiology[J], 2008, 84: 1435—1439 [13]Yu J. H., Choi S., Dickson R. M.. Angew. Chem.[J], 2009, 48: 318—320 [14]Lin C. A. J., Yang T. Y., Lee C. H., Huang S. H., Sperling R. A., Zanella M., Li J. K., Shen J. L., Wang H. H., Yeh H. I., Parak W. J., Chang W. H.. ACS Nano[J], 2009, 3(2): 395—401 [15]Xie J. P., Zheng Y. G., Ying J. Y.. J. Am. Chem. Soc.[J], 2009, 131: 888—889 [16]Hanaki K., Momo A., Oku T., Komoto A., Maenosono S., Yamaguchi Y., Yamamoto K.. Biochemical and Biophysical Research Communications[J], 2003, 302: 496—501

Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	CHEN Weiju, CHEN Shiya, XUE Caoye, LIU Bo, ZHENG Jing. Fluorescent Probe for Hypoxia-triggered Imaging and Cancer Therapy [J]. Chem. J. Chinese Universities, 2021, 42(11): 3433.
[2]	LIU Qianrong,CAI Huiwu,GUAN Ming,QIAO Juan,QI Li. Turn-off Fluorescence of Gold Nanoclusters for Sensing of Ferric Ion^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 55.
[3]	YU Xijuan, HAN Lulu, HUN Xu. Cu²⁺ Modified Gold Nanoclusters for Fluorescence Turn-on Detection of Dopamine^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2169.
[4]	LIU Gui-Feng, GAO Zhong-Li, YU Qiong, ZHANG Hui-Mao, WANG Zhen-Xin. Light Induced Cellular Cytotoxicity of Rich Arginine Peptide Modified Ferric Oxide Core Gold Shell Nanoparticles [J]. Chem. J. Chinese Universities, 2012, 33(09): 1920.
[5]	WANG Li, PENG Xiao-Jun*, DAI Bin, WANG Bing-Shuai, LIU Fei, QIANG Xin-Xin. Novel Water-soluble Asymmetric Cy5 Dyes: Synthesis, Photo-stabilities and Fluorescent Labeling Protein [J]. Chem. J. Chinese Universities, 2010, 31(7): 1381.
[6]	QIAO Xiao-Qiang, ZHANG Lin, LIANG Zhen, ZHANG Wei-Bing, ZHANG Li-Hua*, ZHANG Yu-Kui. Cooperative Effects in the Fluorescent Labeling of Proteins Studied by High-performance Liquid Chromatography [J]. Chem. J. Chinese Universities, 2007, 28(9): 1657.
[7]	WANG Li-Feng, WANG Li-Ping, CHEN Jie, FANG Xue-Xun, LI Wei. Quantum Dots Labeled Immune Peptide and Characterization of the Interaction with T Cell [J]. Chem. J. Chinese Universities, 2006, 27(2): 271.
[8]	LIN Zhang-Bi, SU Xing-Guang, ZHANG Hao, MU Ying, SUN Ye, HU Hai, YANG Bai, YAN Gang-Lin, LUO Gui-Min, JIN Qin-Han . Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling [J]. Chem. J. Chinese Universities, 2003, 24(2): 216.
[9]	LI Fu-Chuan, GENG Mei-Yu, LI Ying-Xia, LI Jing, MIAO Ben-Chun, Guan Hua-Shi. Studies on Fluorescent Labeling of Marine Sulfated Polysaccharide 911 [J]. Chem. J. Chinese Universities, 2002, 23(9): 1704.