Studies on the Interaction for Bovine Serum Albumin with Phenylbutazone and Ibuprofen by Fluorescence Spectrometry

Abstract

Abstract:

The interaction of bovine serum albumin with phenylbutazone and ibuprofen was studied by fluorescence spectrometry. The binding and thermodynamic parameters were obtained. The reasonable results were obtained by programmed calculation process based on the calculating equation established. By comparison with the results obtained by the other calculating methods, it is proved that the satisfactory results can be obtained by the proposed method. In addition, the effects of common ions on the binding of the drugs and bovine serum albumin were also examined.

Key words: Fluorescence, Phenylbutazone, Ibuprofen, Bovine serum albumin, Interaction

TrendMD:

SUN Yan-Tao, ZHANG Yu-Pu, BI Shu-Yun, SUN Ye, LIU He, ZHAI Yu-Juan, ....... Studies on the Interaction for Bovine Serum Albumin with Phenylbutazone and Ibuprofen by Fluorescence Spectrometry[J]. Chem. J. Chinese Universities, 2009, 30(6): 1095.

References

[1]LIU Xue-Feng(刘雪锋), XIA Yong-Mei(夏咏梅), FANG Yun(方云), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2004, 25(11): 2099—2013
[2]LI Lin-Wei(李林尉), WANG Dong-Dong(王冬冬), SUN De-Zhi(孙德志), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2008, 29(6): 1211—1215
[3]Sudlow G., Birkett D. J., Wade D. N.. Mol. Pharmacol.[J], 1975, 11: 824—832
[4]Fehske K. J.,Müller W. E., Wollert U.. Biochem. Pharmacol.[J], 1981, 30: 687—692
[5]Carter D. C., He X. M.. Science[J], 1990, 249: 302—303
[6]He X. M., Carter D. C.. Nature[J], 1992, 358(16): 209—215
[7]XU Yan(徐岩), SHEN Han-Xi(沈含熙), HUANG Han-Guo(黄汉国). Chem. J. Chinese Universities(高等学校化学学报)[J], 1996, 12(7): 1855—1858
[8]Qi Z. D., Zhang Y., Liao F. L., et al.. Journal of Pharmaceutical and Biomedical Analysis[J], 2008, 46: 699—706
[9]Lerman L. S.. J. Mol. Biol.[J], 1961, 3: 18—30
[10]Steiner R. T., Weinryb L.. Excited States of Protein and Nucleic Acid[M], New York: Plenum Press, 1971: 40
[11]CHEN Guo-Zhen(陈国珍), HUANG Xian-Zhi(黄贤智), XU Jin-Gou(许金钩), et al.. Fluorescence Analytical Method, 2nd Ed.(荧光分析法, 第二版), Beijing: Science Press, 1990: 122
[12]Dewey T. G.. Biophysical and Biochemical Aspects of Fluorescence Spectroscopy[M], New York: Plenum, 1991: 1—41
[13]Lakowicz J. R., Webber G.. Biochemistry[J], 1973, 12: 4161—4170
[14]Ware W. R.. J. Phys. Chem.[J], 1962, 66: 455—458
[15]ZHANG Guo-Wen(张国文), GUO Bao-Jin(郭宝金), QUE Qing-Min(阙青民), et al.. Chinese Journal of Analysis Laboratory(分析试验室)[J], 2008, 27(3): 1—4
[16]Mauricio S. B., Guilherme L. I.. J. Phys. Chem.[J], 1998, 102(23): 4678—4688
[17]Feng X. Z.,Lin Z., Yang L. J., et al.. Talanta[J], 1998, 47: 1223—1239
[18]Lakos Z., Szarka A., Koszorus L., et al.. J. Photochem. Photobiol. B: Biol.[J], 1995, 27: 55—60
[19]Yi P. G., Yu Q. S., Shang Z. C., et al.. Acta Pharm. Sinica[J], 2000, 35: 774—777
[20]Bi S. Y., Qiao C. Y., Song D. Q., et al.. Sensors and Actuators B[J], 2006, 119: 199—208
[21]Epps D. E., Raub T. J., Kézdy F. J.. Anal. Biochem.[J], 1995, 227: 342—350
[22]Haq I.. Arch. Biochem. Biophys.[J], 2002, 403: 1—15
[23]MA Gui-Bin(马贵斌), GAO Chi(高尺), REN Bin-Zhi(任斌知). Acta Chimica Sinica(化学学报)[J], 1995, 53(12): 1193—1197
[24]Förster T.. Ann. Phys.[J], 1948, 2: 5—75