Studies on Structure-activity Relationship of 17 Sulfonamides Binding to Antibody by Fluorescence Polarization Immunoassay

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Studies on Structure-activity Relationship of 17 Sulfonamides Binding to Antibody by Fluorescence Polarization Immunoassay

WANG Zhan-Hui, ZHANG Su-Xia, DING Shuang-Yang, SHEN Jian-Zhong*

National Reference Laboratory of Veterinary Drug Residues, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China

Received:2007-11-12 Revised:1900-01-01 Online:2008-06-10 Published:2008-06-10
Contact: SHEN Jian-Zhong

Abstract

Abstract: Fluorescence polarization immunoassay for the determination of sulfamethazine was developed via four polyclonal antibodies against sufamethazine and five fluorescein-labeled sulfonamides(tracers). The study mainly focuses on the relative affinity trend of four polycolnal antibodies toward 17 sulfonamides analogs and explaination of the recognition between antibody and drugs based on the two-dimensional structures of drug antigen. The results demonstrate that the polyclonal antibody(No.3) showed the most sensitivity for sulfamethazine, however, the four polyclonal antibodies presented a similar relative affinity trend to 17 sulfonamides, implying that the affinity of antibody to antigen has correlationship with the chemical structure of antigen.

Key words: Fluorescence polarization immunoassay, Sulfonamide, Polyclonal antibody, Structure-activity relationship

CLC Number:

O657.3

TrendMD:

WANG Zhan-Hui, ZHANG Su-Xia, DING Shuang-Yang, SHEN Jian-Zhong*. Studies on Structure-activity Relationship of 17 Sulfonamides Binding to Antibody by Fluorescence Polarization Immunoassay[J]. Chem. J. Chinese Universities, doi: .

[1]	ZHAO Yingzhe, ZHANG Jianling. Applications of Metal-organic Framework-based Material in Carbon Dioxide Photocatalytic Conversion [J]. Chem. J. Chinese Universities, 2022, 43(7): 20220223.
[2]	YANG Dan, LIU Xu, DAI Yihu, ZHU Yan, YANG Yanhui. Research Progress in Electrocatalytic CO₂ Reduction Reaction over Gold Clusters [J]. Chem. J. Chinese Universities, 2022, 43(7): 20220198.
[3]	HOU Hua, WANG Baoshan. Group Additivity Theoretical Model for the Prediction of Dielectric Strengths of the Alternative Gases to SF₆ [J]. Chem. J. Chinese Universities, 2021, 42(12): 3709.
[4]	YE Xiaodong, QI Guodong, XU Jun, DENG Feng. Glucose Oxidation on Au-supported SBA-15 Molecular Sieve ^† [J]. Chem. J. Chinese Universities, 2020, 41(5): 960.
[5]	CHANG Junpeng,ZHAO Jiarui,CHEN Sijia,MENG Kai,SHI Weini,LI Ruifang. Structure-activity Relationship of Antimicrobial Peptide SAMP1 and Its Analog Peptides^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 705.
[6]	YU Min, HUANG Jingjing, MA Min, FU Ruiyan, YAN Yan, ZHANG Fusheng, YIN Junfeng, XIE Ningning. Zinc Chelating Activity and Quantitative Structure-activity Relationship of Tripeptides^† [J]. Chem. J. Chinese Universities, 2018, 39(2): 234.
[7]	HOU Hua, YU Xiaojuan, ZHOU Wenjun, LUO Yunbai, WANG Baoshan. Theoretical Investigations on the Structure-activity Relationship to the Dielectric Strength of the Insulation Gases^† [J]. Chem. J. Chinese Universities, 2018, 39(11): 2477.
[8]	WANG Lei, ZHENG Guojun, JI Qi, CHEN Bo, GONG Longlong, GAO Congmin, DU Zhenjian, ZHANG Xingmin. Synthesis and Biological Activity of Novel PI3K/mTOR Inhibitors^† [J]. Chem. J. Chinese Universities, 2017, 38(9): 1590.
[9]	LIU Yuming, TIAN Lijun, HU Dong, NIE Jianbing. yntheses and Anti-cholinesterase Activity of 4-N-Phenylaminoquinoline Derivatives ^† [J]. Chem. J. Chinese Universities, 2017, 38(3): 392.
[10]	LIU Benguo, LIU Jiangwei, LI Jiaqi, GENG Sheng, MO Haizhen, LIANG Guizhao. 3D-QSAR and Interaction Mechanism of Flavonoids s P-glycoprotein Inhibitors^† [J]. Chem. J. Chinese Universities, 2017, 38(1): 41.
[11]	GUO Liang, CAO Rihui, FAN Wenxi, GAN Ziyun, MA Qin. Design, Synthesis and in vitro Antitumor Activities of Novel Bivalent β-Carbolines^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1093.
[12]	ZHANG Jie, ZHOU Changjian, XIE Jianwei, DAI Bin. Synthesis and Antitumor Activities of Rhein-Valine Adducts^† [J]. Chem. J. Chinese Universities, 2016, 37(12): 2159.
[13]	XU Jian, WEI Mengxue, LI Guoming, LI Xueqiang. Synthesis and Anti-tumor Activities of Novel Artemisone-piperazine-sulfonamide Derivatives^† [J]. Chem. J. Chinese Universities, 2015, 36(5): 919.
[14]	WU Xianjun, ZHAO Bing, MIAO Xuan, QIAN Cheng, WANG Bingyu, LI Xiaomeng, YANG Baiquan. New Method for Sperm Separation from Mixed Stain Samples^† [J]. Chem. J. Chinese Universities, 2015, 36(5): 886.
[15]	ZHANG Jing, MU Boshuai, WU Meng, BIAN Yanqing, LI Yuan. Synthesis, Antifungal Activity and Structure-activity Relationship of -Fluorophenyl-2,3-dihydro-1,5-benzothiazepines Derivatives^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 687.

Studies on Structure-activity Relationship of 17 Sulfonamides Binding to Antibody by Fluorescence Polarization Immunoassay

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments