Abstract: A novel brush-type chiral stationary phase(CSP-a) for HPLC was prepared by surface-initiated polymerization with (R)-(+)-1,1'-binaphthyl-2,2'-diamine as a chiral monomer and dichloride of 4,4'-azobis-4-cyanopentanoic acid bonded on the surface of silica gel as a radical initiator. The resolutions of the enantiomers of binaphthol derivatives, N-3,5-dinitrobenzoyl-D,L-amino acid methyl esters and isopropyl esters, and a trans-ethylene oxide derivative on CSP-a were carried out using heptane-alcohol-organic modifier-organic acid as the mobile phases under the detection conditions of the column temperature of 30 ℃ and UV wavelength of 254 nm. The effects of organic modifier and organic acid on the resolutions of the analytes on CSP-a were examined. The results show that CSP-a prepared was effective for the resolutions of the enantiomers of π-acid and π-base derivatives. Trifluoroacetic acid was a better organic modifier than acetic acid. Adding CH₂Cl₂ and CHCl₃ to the mobile phase could increase retention and resolution of the samples on CSP-a.

Key words: Chiral stationary phase, 1,1’-Binaphthyl-2,2’-diamine, Enantiomeric resolution, Binaphthol derivative, DNB-amino acid ester, Ethylene oxide derivative