Studies on Binding Equilibrium Between As(Ⅲ)/As(Ⅴ) and BSA via Dialysis and High Performance Liquid Chromatography-Hydride Generation On-Line Coupled with Atomic Fluorescence Spectrometry

Chem. J. Chinese Universities

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Studies on Binding Equilibrium Between As(Ⅲ)/As(Ⅴ) and BSA via Dialysis and High Performance Liquid Chromatography-Hydride Generation On-Line Coupled with Atomic Fluorescence Spectrometry

JIANG Hong*, DING Jing-Hua, ZHANG Ying-Hua, GONG Hui-Zhi, GAO Shuang, SUN Gui-Fan

School of Public Health, China Medical University, Shenyang 110001, China

Received:2007-07-10 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10
Contact: JIANG Hong

Abstract

Abstract: The binding equilibrium of As(Ⅲ)/As(Ⅴ) to bovine serum albumin(BSA) was studied by Dialysis and High Performance Liquid Chromatography-hydride generation on-line coupled with atomic fluorescence spectrometry(D-HPLC-HG-AFS) at physiological(pH=7.4). The binding plots analysis indicates that the binding model is Scatchard when the concentrations of As(Ⅲ) ions are lower[c_As(Ⅲ)∶c_BSA≤1∶1], there is 1.4 strong binding site of As(Ⅲ) ions in BSA, the stability constants is 1.7×10⁶ at physiological(pH=7.4). The binding model is Plasvento when the concentrations of As(Ⅲ) ions are higher(c_As(Ⅲ)∶c_BSA≥2∶1], and As(Ⅴ) ions has litter effect on BSA. The method based on HPLC-HG-AFS for arsenic speciation analysis was developed. The factors of the method including HPLC mobile phase, concentrations of HCl, flow rate of HCl, concentration and flow rate of KBH₄ were investigated in this paper. Under the optimum conditions, the detection limits for As(Ⅲ) and As(Ⅴ) were 2.89, 6.38 ng/L, respectively.

Key words: As(Ⅲ), As(Ⅴ), Bovine serum albumin, Dialysis, High performance liquid chromatography-hydride generation on-line coupled with atomic fluorescence spectrometry

CLC Number:

O656.5

TrendMD:

JIANG Hong*, DING Jing-Hua, ZHANG Ying-Hua, GONG Hui-Zhi, GAO Shuang, SUN Gui-Fan. Studies on Binding Equilibrium Between As(Ⅲ)/As(Ⅴ) and BSA via Dialysis and High Performance Liquid Chromatography-Hydride Generation On-Line Coupled with Atomic Fluorescence Spectrometry[J]. Chem. J. Chinese Universities, doi: .

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Studies on Binding Equilibrium Between As(Ⅲ)/As(Ⅴ) and BSA via Dialysis and High Performance Liquid Chromatography-Hydride Generation On-Line Coupled with Atomic Fluorescence Spectrometry

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