Novel Fluorescent Method of Protein Detection Using Hairpin Nucleic Acid Aptamer Based on Polymerase Reaction

Chem. J. Chinese Universities

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Novel Fluorescent Method of Protein Detection Using Hairpin Nucleic Acid Aptamer Based on Polymerase Reaction

GUO Qiu-Ping, YANG Xiao-Hai, WANG Ke-Min*, MENG Xiang-Xian, LI Jun, TAN Wei-Hong

State Key of Laboratory of Chemo/biosensing and Chemomertrics, Biomedical Engineering Center, Institute of Biological Science and Technology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China

Received:2007-06-05 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
Contact: WANG Ke-Min

Abstract

Abstract: Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity to targets. In this paper, a novel fluorescent method of protein detection was developed via hairpin aptamer based on polymerase reaction. The hairpin aptamer was designed as protein ligand and template of the polymerase reaction. When the aptamer was bound to the target protein, it would change to a linear strand and induce the polymerase reaction. Then protein detection was carried out by monitoring the polymerase reaction without directly labeling with the aptamer, with a linear range of 0.5—8 nmol/L and detection limit of 0.5 nmol/L. This proposed method has a potential advantage to design other protein probe with linear aptamer with a complex structure and can be used as a simple and general tool for protein detection.

Key words: Aptamer, Protein detection, Polymerase reaction, Thrombin

CLC Number:

O657
O629.73

TrendMD:

GUO Qiu-Ping, YANG Xiao-Hai, WANG Ke-Min*, MENG Xiang-Xian, LI Jun, TAN Wei-Hong. Novel Fluorescent Method of Protein Detection Using Hairpin Nucleic Acid Aptamer Based on Polymerase Reaction[J]. Chem. J. Chinese Universities, doi: .

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Novel Fluorescent Method of Protein Detection Using Hairpin Nucleic Acid Aptamer Based on Polymerase Reaction

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