Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (3): 424.

• Articles • Previous Articles     Next Articles

Fluorescent Characterization of Peroxynitrite-induced Thiyl  Radicals

DANG Ya-Min,GUO Xiang-Qun*   

  1. Department of Chemistry,the Key Laboratory of Analytical Science of MOE,
     Xiamen University,Xiamen 361005,China
  • Received:2005-06-27 Online:2006-03-10 Published:2006-03-10

Abstract:

A spin-labeling fluorophore(Ac-Tempo·),formed by combining with an acridine with a paramagnetic nitroxide(4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1-oxy),was explored for peptide-based thiyl radicals generated through the reaction between gluththione and peroxynitrite. Ac-Tempo·,a weak fluorescent compound,showed a dramatic increase in fluorescence intensity after interaction with thiyl radicals,and could be employed to probe peptide-based thiyl radicals.

Key words: Spin-labeling; Fluorescent probe; Thiyl Radical; Peroxynitrite

CLC Number: 

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