Abstract: The metabolite profiles from rat hepatic fibrosis were investigated by using high-resolution magic-angle spinning ¹H nuclear magnetic resonance(HR-MAS NMR) and principal component analysis(PCA) on the intact tissue. The metabolite characters of hepatic fibrosis were directly observed on ¹H NMR spectra by the elevated level of lipids, depleted levels of glucose, glycogen, taurine and inositol, and the decreased levels of choline(Cho) and glycerophosphocholine(GPC) compared with that of phosphocholine(PC). There was a dramatic tendency observed for the level of lipids from the normal to the aggravated stages of hepatic fibrosis, which ascended greatly at first and descended a little bit at the last stage. However, no evident changes of other metabolites in different hepatic fibrosis stages were observed. The results of PCA manifested that hepatic fibrosis tissue samples were separated well from those of the control and no obvious diversities were observed among the samples at different hepatic fibrosis stages.

Key words: HR-MAS NMR, Principal component analysis(PCA), Metabolite, Hepatic fibrosis