Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (5): 835.

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Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography

YAN Li-Juan^1,2, ZHANG Qing-He^1,3, ZHANG Wei-Bing¹, FENG Yu-Qi², ZHANG Li-Hua¹, LI Tong^1,3, ZHANG Yu-Kui¹

1. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
3. Dalian Elite Analytical Instruments Co. Ltd., Dalian 116011, China

Received:2004-11-10 Online:2005-05-10 Published:2005-05-10

Abstract

Abstract: A silica-bonded bovine serum albumin(BSA) chiral monolithic stationary phase for capillary electrochromatography(CEC) was introduced. An inorganic-organic hybrid monolithic column was firstly prepared by sol-gel chemistry with homogeneously distributed aminopropyl groups throughout the silica matrix. Then the chiral stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde. The effects of pH value and concentration of phosphate buffer on the separation of D,L-tryptophan were investigated. The separation factor of D,L-tryptophan reached 3.37 on CEC mode.

Key words: Capillary electrochromatography(CEC), Monolithic column, Sol-gel, Chiral stationary phases(CSPs), Bovine serum albumin(BSA)

CLC Number:

O658

TrendMD:

YAN Li-Juan, ZHANG Qing-He, ZHANG Wei-Bing, FENG Yu-Qi, ZHANG Li-Hua, LI Tong, ZHANG Yu-Kui . Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography[J]. Chem. J. Chinese Universities, 2005, 26(5): 835.

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Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography

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