Construction of a New Method for Mobilizing the Zones of CIEF and Its Application

Abstract

Abstract: A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.

Key words: Capillary isoelectric focusing(CIEF), Electroosmotic flow(EOF), Protein, Polypeptide

CLC Number:

O658

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ZHU Gui-Jie, YANG Chun, LIU He-Chun, ZHANG Li-Hua, ZHANG Wei-Bing, ZHANG Yu-Kui. Construction of a New Method for Mobilizing the Zones of CIEF and Its Application[J]. Chem. J. Chinese Universities, 2005, 26(4): 654.

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Construction of a New Method for Mobilizing the Zones of CIEF and Its Application

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