Starch Nanoparticle as Transgenic Vehicle Mediated by Ultrasound

Abstract

Abstract: A method in which poly-L-lysine-starch nanoparticle(PLL-StNP) could act as gene vehicle mediated by ultrasound was described in this paper. It was found that when pIRGFP plasmid DNA-PLL-StNP complexes were treated with ultrasound for different time, electrophoresis analysis showed that the biological characteristics of DNA could be well-kept, when DNA-PLL-StNP complexes were transferred into COS-7 cells mediated by ultrasound for 2 min at 120 W and 40 kHz, the cells were expressed GFP at a frequency of about 70. This method may(will) provide a wide application not only in animal transgenic technique and in human gene therapy, but also in plant transgenic technique.

Key words: Nanoparticle, Gene vehicle, Ultrasound, Gene transfer

CLC Number:

O629.73

TrendMD:

LIU Jun, LIU Xuan-Ming, XIAO Su-Yao, TONG Chun-Yi, TANG Dong-Ying, ZHAO Li-Jian. Starch Nanoparticle as Transgenic Vehicle Mediated by Ultrasound[J]. Chem. J. Chinese Universities, 2005, 26(4): 634.

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Starch Nanoparticle as Transgenic Vehicle Mediated by Ultrasound

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