Establishment and Evaluation of Comprehensive Two-dimensional Liquid Chromatography(IEC/RP)

Abstract

Abstract: A two-dimensional liquid chromatography is established with ion-exchange chromatography(IEC) followed by reversed phase liquid chromatography(RP). The interface is a ten-port two-position switching valve coupled with two parallel RP columns. After splitting the IEC effluent with a ratio of 10:1(volume ratio), the IEC profile and the RP profile were obtained simultaneously. The system is evaluated with 5 standard proteins, which cannot be resolved in either of IEC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 189.

Key words: Comprehensive two-dimensional liquid chromatography, Protein, Peak capacity

CLC Number:

O658

TrendMD:

WANG Zhi-Cong, ZHANG Qing-He, ZHANG Wei-Bing, LI Tong, ZHAO Zhong-Yi . Establishment and Evaluation of Comprehensive Two-dimensional Liquid Chromatography(IEC/RP)[J]. Chem. J. Chinese Universities, 2005, 26(3): 426.

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