Application of Capillary Enzyme Column Enhancd by Using Nanoparticles for Glucose Biosensor Based on Liquid Droplets

Abstract

Abstract: An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO₂ nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.

Key words: Nanoparticle, Capillary enzyme column, Liquid drop sensor, Fluorometry

CLC Number:

O657

TrendMD:

FENG Feng, WANG Ke-Min, CHEN Qi-Ting, XIANG Qiu-Fen, CHEN Ze-Zhong, HUANG Hong-Mei, HUANG Shan-Sheng, HUO Xi-Qin. Application of Capillary Enzyme Column Enhancd by Using Nanoparticles for Glucose Biosensor Based on Liquid Droplets[J]. Chem. J. Chinese Universities, 2004, 25(7): 1257.

[1]	Jinhan Sheng, Qizhen Zheng, Ming Wang. Non-viral delivery of CRISPR/Cas9 Genome Editing [J]. Chem. J. Chinese Universities, 2022, 43(Album-4): 20220344.
[2]	YANG Jingyi, LI Qinghe, QIAO Botao. Synergistic Catalysis Between Ir Single Atoms and Nanoparticles for N₂O Decomposition [J]. Chem. J. Chinese Universities, 2022, 43(9): 20220388.
[3]	LIU Shuwei, JIN Hao, YIN Wanzhong, ZHANG Hao. Gemcitabine/polypyrrole Composite Nanoparticles for Chemo-photothermal Combination Ovarian Cancer Therapy [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220345.
[4]	WANG Guangqi, BI Yiyang, WANG Jiabo, SHI Hongfei, LIU Qun, ZHANG Yu. Heterostructure Construction of Noble-metal-free Ternary Composite Ni（PO₃）₂-Ni₂P/CdS NPs and Its Visible Light Efficient Catalytic Hydrogen Production [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220050.
[5]	JI Fa, LIU Ling, YU Linling, SUN Yan. Effects of Muco-inert and Acid-sensitive Modification on Mucosal Penetration of Nanoparticles [J]. Chem. J. Chinese Universities, 2022, 43(6): 20210837.
[6]	WANG Hong, SAN Khin Nyein Ei, FANG Yun, ZHANG Xinyu, FAN Ye. Pickering Emulsion Stabilization and Interfacial Catalytic Oxidation by Janus Nano-Au [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220105.
[7]	SUN Xuefeng, RENAGUL Abdurahman, YANG Tongsheng, YANG Qianting. Synthesis and Luminescence Properties of Cr，In Co-doped Small Size MgGa₂O₄ Near-infrared Persistent Luminescence Nanoparticles [J]. Chem. J. Chinese Universities, 2022, 43(4): 20210850.
[8]	TAO Xingfu, HAN Chenglong, YANG Yang, LIU Kun. Synthesis of Aluminum Nanoparticles@Polymer Core-shell Nanostructures by Surface-initiated Polymerization [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220367.
[9]	YANG Zhaohua, CHENG Hongjing, YANG Yi, LIU Hui, DU Feipeng, ZHANG Yunfei. Preparation of Silver-loaded Polyvinyl Alcohol Sponge and Its Interfacial Photothermal Driven Water Evaporation Performance [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220181.
[10]	CHU Mingyue, LI Fengbo, GAO Ning, YANG Xin, YU Tingting, MA Huiyuan, YANG Guixin, PANG Haijun. Construction of a Coronal Polyoxometalate-based Composite Film for Determination of Nitrite [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210579.
[11]	WANG Zhaoyi, MU Shilin, ZHANG Xuemin, ZHANG Junhu. Fabrication and Optical Mechanism of Plasmonic Nanodimer with Uniform Orientation [J]. Chem. J. Chinese Universities, 2021, 42(8): 2374.
[12]	CHEN Shaoyun, ZHANG Xingying, LIU Ben, TIAN Du, LI Qi, CHEN Fang, HU Chenglong, CHEN Jian. Controllable Growth of Silver Nanoparticles on TiO₂ Tetragonal Prism Nanarrays and Its SERS Effect [J]. Chem. J. Chinese Universities, 2021, 42(8): 2381.
[13]	WANG Yawen, LI Dong, LIANG Wenkai, SUN Yinghui, JIANG Lin. Multiplex Structures of Plasmonic Metal Nanoparticles and Their Applications [J]. Chem. J. Chinese Universities, 2021, 42(4): 1213.
[14]	ZHENG Haijiao, JIANG Liyan, JIA Qiong. Arginine Functionalized Magnetic Nanoparticles and Its Application in Phosphopeptides Enrichment [J]. Chem. J. Chinese Universities, 2021, 42(3): 717.
[15]	LIU Zhigang, LI Jiabao, YANG Jian, MA Hao, WANG Chengyin, GUO Xin, WANG Guoxiu. Preparation of a Novel g-C₃N₄/Sn/N-doped Carbon Composite for Sodium Storage [J]. Chem. J. Chinese Universities, 2021, 42(2): 633.

Application of Capillary Enzyme Column Enhancd by Using Nanoparticles for Glucose Biosensor Based on Liquid Droplets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments