Preparation of Nano-scaled Magnetic Biological Probes of Fe3O4/Dextran/Antibody and Chromatographic Assay

Abstract

Abstract: Dextran coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.

Key words: Magnetic biological probes, Antigen antibody recognition, BSA Blocking, Chromatographic assay

CLC Number:

O657

TrendMD:

HONG Xia, GUO Wei, LI Jun, LIU Yan-Mei, YANG Wen-Sheng, BAI Yubai, LI Tie-Jin , YUAN Hang, MA Lan. Preparation of Nano-scaled Magnetic Biological Probes of Fe₃O₄/Dextran/Antibody and Chromatographic Assay[J]. Chem. J. Chinese Universities, 2004, 25(3): 445.

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Preparation of Nano-scaled Magnetic Biological Probes of Fe₃O₄/Dextran/Antibody and Chromatographic Assay

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