Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes

Abstract

Abstract: An amphiphile, 9-octanyl adenine trimethylammonium bromide(OATMAB amphiphile 3), was synthesized as the receptor. The coliposomes were prepared from phosphatidylcholine and 9-octyl adenine trimethylammonium bromide by sonication of CHCl₃-cast films of amphiphiles mixtures [n(PC): n(MOATMAB)=10:1] in doubly distilled water with a bath-type sonicator at 80 W for 3 h. As thymine was added into PC/MAOTMAB coliposomal solution, the absorbability of thymine at 263 nm decreased slowly with time change from 0 to 72 h. The UV-Vis spectroscopic behavior of the thymine in PC/OATMAB/thymine coliposomes(molar ratio=10:1:1) was slowly decreaced with the increase of time. The onset of a hypochromic effect can be ascribed to adenine-thymine interaction. The absorption band levels off at 45.4% of its initial value and stabilizes after several weeks.

Key words: Nucleobases, Molecular recognition, Amphiphile, Coliposomes

CLC Number:

O641.3

TrendMD:

SONG Li-Ming, LING Xing, LI Guo-Wen . Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes[J]. Chem. J. Chinese Universities, 2004, 25(12): 2356.

[1]	WANG Longjie, FAN Hongchuan, QIN Yu, CAO Qiue, ZHENG Liyan. Research Progress of Metal-organic Frameworks in the Field of Chemical Separation and Analysis [J]. Chem. J. Chinese Universities, 2021, 42(4): 1167.
[2]	ZHANG Xiaorong, CHEN Lanlan, HU Shanwen. Advances in Bacteria Biosensing Based on Molecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3468.
[3]	JI Cailing, CHENG Xing, TAN Jie, YUAN Quan. Selection of Functionalized Aptamers and Their Applications in Molecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3457.
[4]	LIU Xuejiao, YANG Fan, LIU Shuang, ZHANG Chunjuan, LIU Qiaoling. Progress in Aptamer-targeted Membrane Protein Recognition and Functional Regulation [J]. Chem. J. Chinese Universities, 2021, 42(11): 3277.
[5]	LIU Yuan, DENG Jinqi, ZHAO Shuai, TIAN Fei, LI Yi, SUN Jiashu, LIU Chao. Lateral Flow Assay Based on Molecular Recognition for Diagnosis of Corona Virus Disease 2019 Infection [J]. Chem. J. Chinese Universities, 2021, 42(11): 3390.
[6]	LIN Ningqin, YAO Ke, CHEN Xiangjun. Research Progress of Molecular Recognition and Interaction of Crystallins Linking Cataract [J]. Chem. J. Chinese Universities, 2021, 42(11): 3379.
[7]	HUANG Ling, ZHUANG Zijian, LI Xiang, SHI Muling, LIU Gaoqiang. Advances in Molecular Recognition of Exosomes Based on Aptamers [J]. Chem. J. Chinese Universities, 2021, 42(11): 3493.
[8]	MA Yucong,FAN Baomin,HAO Hua,LÜ Jinyu,FENG Yunhao,YANG Biao. Experimental and Theoretical Studies of Action Mechanism of an Octadecylamine-based Molecular Assembly on Mild Steel^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 96.
[9]	SHEN Xiaoqin, LI Zhi, WANG Ganglin, WANG Li, SUN Quanhong, LUO Xucheng, MA Nan. Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2176.
[10]	SONG Chunxia, YANG Xiaohai, WANG Kemin, WANG Qing, LIU Jianbo, HUANG Jin, LI Wenshan, HUANG Haihua, LIU Wei. Application of Polymers in Fluorescence Analysis^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 201.
[11]	ZHOU Ting, CAO Zhong, DAI Yun-Lin, CAO Ting-Ting, HE Jing-Lin, XU Lei-Tao, LONG Shu. Sensing Mechanism and Application for Recognition of Ethanol by Calixarene Supramolecules Based on Hydrogen Bonds Interaction [J]. Chem. J. Chinese Universities, 2013, 34(6): 1339.
[12]	KOU Ying-Ying, MENG Qing-Bin, LIU Ke-Liang. Coassembly of Palmitic Acid Modified α-Helical Peptide Amphiphiles [J]. Chem. J. Chinese Universities, 2012, 33(11): 2476.
[13]	WANG Kai, YANG Ying-Wei, ZHANG Sean Xiao-An. Research Progress on the Synthesis of Pillar[n]arenes and Their Host-guest Chemistry [J]. Chem. J. Chinese Universities, 2012, 33(01): 1.
[14]	LI Guang-Quan, YU Jing-Sheng, LI Guo-Wen*. Novel Fluorescent Vesicular Sensor for Saccharides Based on Boronic Acid\\|diol Interaction [J]. Chem. J. Chinese Universities, 2011, 32(3): 793.
[15]	YUAN Fei, LI Qiu-Yue, WANG Wei*. Synthesis of Amphiphilic Block Codendrimers Gx-PBE-b-Gx-PMPA(OH)x2 and Study on Temperature Dependence of Multiple Hydrogen Bonds [J]. Chem. J. Chinese Universities, 2011, 32(11): 2667.

Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments