Isolation and Structural Analysis of the Polysaccharides of Limonium bicolor and the Inhibition to Hela Cell

Abstract

Abstract: Soluble polysaccharides were isolated from Limonium bicolor Kunge(Bge.) and purified by conventional method. IR, GC-MS, ¹³C NMR and ¹H NMR were used to analyze the structures of the polysaccharides. The result showed that glucose and glucose acid were the main components of the polysaccharides, and the bond between GlcA and Glc was α-(1→6), then the disaccharide unit was connected by α-(1→4) linkage. The polysaccharides were fluorescent with the biggest emission peak at 460 nm, and Zn²⁺ or Ca²⁺ enhanced the fluorescence intensity of LP. Much more research proved that LP had the function of inhibiting the growth of Hela cells, with the IC₅₀ being 62.2 μg/mL.

Key words: Limonium bicolor, Polysaccharides, Structure, Hela cells, IC₅₀

CLC Number:

O629.2

TrendMD:

ZHANG Lian-Ru, CHEN Ka-Lin, LI Ni, ZOU Guo-Lin . Isolation and Structural Analysis of the Polysaccharides of Limonium bicolor and the Inhibition to Hela Cell[J]. Chem. J. Chinese Universities, 2004, 25(11): 2034.

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