A Novel Method for Quaternization of Amino Acid/Peptide and Its Product Detection Using Electrospray Ionization Mass Spectrometry

Abstract

Abstract: A novel detection method for quaternization of amino acid and peptide was reported. In this method Ag₂CO₃ was employed instead of KHCO₃ to catalyze the occurrence of quaternization reaction. The absence of metal ions in the reaction system enabled us to in situ detect the quaternizing products by using electrospray ionization mass spectrometry(ESI-MS). We analyzed quaternizing products of six amino acids and two peptides in situ, and obtained the ratio of major product to by-product, which suggests the new method possesses prominent advantages over the old one. This method could offer a potential chance to combine quaternization with mass spectrometry which is helpful for the future use of quaternization reaction in quantitative proteomics.

Key words: Electrospray ionization mass spectrometry, Amino acid/peptide, Quaternization

CLC Number:

O657.7

TrendMD:

BAO Hui-Min, ZHAI Jian-Jun, ZHANG Guo-An, YANG Peng-Yuan, CHEN Xian . A Novel Method for Quaternization of Amino Acid/Peptide and Its Product Detection Using Electrospray Ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, 2004, 25(11): 2013.

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A Novel Method for Quaternization of Amino Acid/Peptide and Its Product Detection Using Electrospray Ionization Mass Spectrometry

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