Determination of High Polymerization Degree Dextran by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry(MALDI -TOFMS) and Comparison of Matrix Effects in the Determination

Abstract

Abstract: A dextran sample with a high polymerization degree(n>100) was directly determined by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOFMS) without being further fractionated and the molecular weight of the dextran up to more than 18 000 had been detected. The effects of matrixes 2,5-dihydroxybenzoic acid(DHB), 1-hydroxyisoquinoline(1-HIQ), 3,4-dihydroxycinnamic acid(caffeic acid, CA) and binary matrixes DHB+1-HIQ, DHB+CA were tested and compared. The results showed that the larger mass range could be obtained when matrix DHB was used, the dextran molecules were desorbed and ionized more efficiently and the reproducibility of spectra was enhanced when binary matrixes DHB+ 1-HIQ and DHB+CA were used, also the resolution of spectra were increased when binary matrixes DHB+1-HIQ were used.

Key words: MALDI-TOF-MS, Dextran, Matrix

CLC Number:

O657.6

TrendMD:

DENG Hui-Min, ZHANG Zhen-Ying, ZHA Qing-Min, DENG Qin-Ying, ZHAO Shan-Kai . Determination of High Polymerization Degree Dextran by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry(MALDI -TOFMS) and Comparison of Matrix Effects in the Determination[J]. Chem. J. Chinese Universities, 2003, 24(6): 996.

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Determination of High Polymerization Degree Dextran by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry(MALDI -TOFMS) and Comparison of Matrix Effects in the Determination

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