Research on the Stability of Relative Strength of Sub-ion in ESI-MS-MS Analysis

Abstract

Abstract: The work presents an electrospray tandem mass spectrometric(ESI-MS-MS) method capable of validating the stability of the product ion ratios of alkaloids at the optimal collision energy(OCE). The mass spectrometer was operated in MS-MSmultiple reaction monitoring(MRM) mode, monitoring four reactions of one alkaloid. The product ion ratios are specific and reproducible(relative standard deviation: <6%, the percent deviation between two different matrixes: <4.5%). This unreported result shows the ion ratios at OCE are stable and independent of the analyte concentration and matrix. This described method can be used in the qualitative analysis in the complex matrix.

Key words: Sub-ion ion, Tandem mass spectrometry, Multiple reaction monitoring

CLC Number:

O658

TrendMD:

SUN Ping, LUO Guo-An, QU Jun . Research on the Stability of Relative Strength of Sub-ion in ESI-MS-MS Analysis[J]. Chem. J. Chinese Universities, 2003, 24(12): 2169.

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Research on the Stability of Relative Strength of Sub-ion in ESI-MS-MS Analysis

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