Separation of N-(2-Ethyl-6-methylphenyl)alanine Enantiomers by Capillary Electrophoresis

Abstract

Abstract: Capillary electrophoresis was used for the chiral separation of N-(2-ethyl-6-methylphenyl)alanine enantiomers in this paper. Capillary zone electrophoresis conditions were used with β-cyclodextrin or its derivatives as a chiral mobile phase additive. The effects of cyclodextrin type, variation of cyclodextrin concentration, background electrolyte pH, temperature and field strength were investigated. Optimum separation was achieved for N-(2-ethyl-6-methylphenyl)alanine enantiomers by using 2,6-di-O-methyl-β-cyclodextrin(40 mmol/L), the temperature(20 ℃) and pH(5.5). The mass concentration range from 20 to 200 mg/Lproved to be sufficient for exact quantification. The detection limitation is 10 mg/L. Anew method of using capillary electrophoresis for the chiral separation of N-(2-ethyl-6-methylphenyl)alanine enantiomers has been established.

Key words: Capillary electrophoresis, Chiral separation, N-(2-Ethyl-6-methylphenyl)alanine

CLC Number:

O658

TrendMD:

ZHENG Liang-Yu, ZHANG Suo-Qin, GAO Gui, CAO Shu-Gui, ZHANG Gui-Rong, WANG Zhi, HAN Si-Ping, QUAN Jing . Separation of N-(2-Ethyl-6-methylphenyl)alanine Enantiomers by Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2003, 24(12): 2165.

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Separation of N-(2-Ethyl-6-methylphenyl)alanine Enantiomers by Capillary Electrophoresis

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