Preparation and Characterization of Polyurethane Molecular Stamps

Abstract

Abstract: Polyurethane based on polypropylene glycol(PPG) and TDIusing 3,3'-dichloride-4,4'-methylenedianiline(MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps(PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and photoresist motherboard showed that PUelastomer could replicate subtly the motherboard's patterns with a high fidelity. It was proved from the contact angle measurement that PU stamps surface has a good affinity with acetonitrile, which guarantee the well-distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirm that polyurethane is an excellent material for molecular stamps.

Key words: Molecular stamps, Polyurethane, Soft lithography, DNA Microarray

CLC Number:

O63
Q503

TrendMD:

LIU Zheng-Chun, HE Quan-Guo, XIAO Peng-Feng, LIANG Bo, HE Nong-Yue, LU Zu-Hong. Preparation and Characterization of Polyurethane Molecular Stamps[J]. Chem. J. Chinese Universities, 2002, 23(9): 1823.

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Preparation and Characterization of Polyurethane Molecular Stamps

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