The Interaction of Human Lipoprotein with Cholesterol Modified Dextran in Serum

Abstract

Abstract: The interaction of human low-density lipoprotein(LDL) and very-low-density lipoprotein(VLDL) with cholesterol modified dextran(CHD) in serum was studied by turbidity and high performance liquid chromatography(HPLC)method. The solution turbidity of VLDLreacting with CHDwas higher than that of LDLwith CHDand was influenced by the concentration of CHD, cholesterol content of CHDand molecular weight of CHD. The turbidity reached the maximum value when the concentration of CHDwas 0.3 mg/mLand cholesterol content was 4 cholesterol group/100 dextran glucose units. Ca ²⁺ had no influence on the interaction of VLDLand LDLwith CHD.The molecular weights of LDL CHDcomplex were monitored by HPLCwhich showed that CHDmolecules could associate multi molecules of LDLcorrelating with the results of turbidity experiments. The HPLCexperiments showed that when cholesterol substitution number was 4/100 glucose units, the interaction of LDLwith CHDreached the optimal condition and the complexation molecular weight increased with increasing dextran molecular weight. The experimental results show that CHDcan induce self-aggregation of LDLor VLDL.

Key words: Interaction, Low density lipoprotein, Very low-density lipoprotein, Cholesterol modified dextran

CLC Number:

Q513

TrendMD:

CAO Ning-Ning, YU Yao-Ting, WANG Man-Yan, WANG Lian-Yong, CHEN Chang-Zhi. The Interaction of Human Lipoprotein with Cholesterol Modified Dextran in Serum[J]. Chem. J. Chinese Universities, 2002, 23(8): 1524.

[1]	REN Shijie, QIAO Sicong, LIU Chongjing, ZHANG Wenhua, SONG Li. Synchrotron Radiation X-Ray Absorption Spectroscopy Research Progress on Platinum Single-atom Catalysts [J]. Chem. J. Chinese Universities, 2022, 43(9): 20220466.
[2]	ZHOU Leilei, CHENG Haiyang, ZHAO Fengyu. Research Progress of CO₂ Hydrogenation over Pd-based Heterogeneous Catalysts [J]. Chem. J. Chinese Universities, 2022, 43(7): 20220279.
[3]	PENG Kuilin, LI Guilin, JIANG Chongyang, ZENG Shaojuan, ZHANG Xiangping. Research Progress for the Role of Electrolytes in the CO₂ Electrochemical Reduction [J]. Chem. J. Chinese Universities, 2022, 43(7): 20220238.
[4]	HU Huimin, CUI Jing, LIU Dandan, SONG Jiaxin, ZHANG Ning, FAN Xiaoqiang, ZHAO Zhen, KONG Lian, XIAO Xia, XIE Zean. Influence of Different Transition Metal Decoration on the Propane Dehydrogenation Performance over Pt/M-DMSN Catalysts [J]. Chem. J. Chinese Universities, 2022, 43(4): 20210815.
[5]	LIU Yang, LI Wangchang, ZHANG Zhuxia, WANG Fang, YANG Wenjing, GUO Zhen, CUI Peng. Theoretical Exploration of Noncovalent Interactions Between Sc₃C₂@C₈₀ and ［12］Cycloparaphenylene Nanoring [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220457.
[6]	JIANG Shan, SHEN Qianqian, LI Qi, JIA Husheng, XUE Jinbo. Pd-loaded Defective TiO₂ Nanotube Arrays for Enhanced Photocatalytic Hydrogen Production Performance [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220206.
[7]	XU Wenzhe, ZHANG Hao. Supramolecular Interactions-mediated Nanodrug Nucleation [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220264.
[8]	PAN Xiaojun, BAO Rongrong, PAN Caofeng. Research Progress of Flexible Tactile Sensors Applied to Wearable Electronics [J]. Chem. J. Chinese Universities, 2021, 42(8): 2359.
[9]	WEN Wei, HUANG Dading, BAO Jingxiao, ZHANG John Z. H.. Residue Specific Binding Mechanisms of PD-1 to Its Monoclonal Antibodies by Computational Alanine Scanning [J]. Chem. J. Chinese Universities, 2021, 42(7): 2161.
[10]	ZHANG Jun, LIU Yixuan, DU Xiaohui, YANG Hui. Highly Adhesive and Stretchable Polymers for the Interface of Cyber-human Interaction [J]. Chem. J. Chinese Universities, 2021, 42(4): 1093.
[11]	MA Zhuoyuan, WANG Dayang. Status and Prospect of Surface Wettability of Molecular Self-assembled Monolayers [J]. Chem. J. Chinese Universities, 2021, 42(4): 1031.
[12]	WANG Yupeng, ZHAO Yang, LI Mohan, SHI Suqing, GONG Yongkuan. Fabrication of Antifouling-antibacterial Dual Functional Polymer Coating via Dopamine-based Multiple Interactions [J]. Chem. J. Chinese Universities, 2021, 42(3): 811.
[13]	LI Mengshuo, ZHANG Jing, LIU Dan, ZHU Yaxian, ZHANG Yong. Interactions of Pyrene with Human Serum Albumin and Bovine Serum Albumin： Microenvironmental Polarity Differences at Binding Sites [J]. Chem. J. Chinese Universities, 2021, 42(3): 731.
[14]	LI Xiaolei, SUN Yunjiao, TANG Ying, WANG Changsheng. Rapid and Accurate Calculation of the Three⁃body Interaction Strength in the Hydrogen⁃bonded Complexes of Alcohols or Deoxyribose with Water [J]. Chem. J. Chinese Universities, 2021, 42(12): 3664.
[15]	CHEN Binggang, LIU Sanrong, YU Xifei, JIANG Zijiang. Preparation and Properties Characterization of Polysiloxane Skin Tissue Adhesive [J]. Chem. J. Chinese Universities, 2021, 42(12): 3746.

The Interaction of Human Lipoprotein with Cholesterol Modified Dextran in Serum

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments