NMR Study of ATP Binding Site with Cd2+

Abstract

Abstract: In this paper, the binding site of Cd²⁺ at the purine ring of ATPwas studied by using NMRtechnique.The changes of :H, 15Nand 31Pchemical shifts and 31P-31Pcoupling constants due to the presence of Cd²⁺ and the change of ¹¹³Cd chemical shift due to the presence of ATPin a wide pHrange are measured.It is found that, when pH>4.5, ATPchelates Cd²⁺ with its phosphate and N7; at pH 2.5—4.5, ATP binding sites are mainly phosphate and Nl, but phosphate/N7 mode still exists; when pHis very low (pH<2.5), ATPdoes not interact with Cd²⁺.

Key words: ATP-Cd²⁺ complex, pH, Chemical shift, Binding site

CLC Number:

O657

TrendMD:

JIANG Ling, MAO Xi-An. NMR Study of ATP Binding Site with Cd²⁺[J]. Chem. J. Chinese Universities, 2002, 23(5): 763.

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NMR Study of ATP Binding Site with Cd²⁺

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