Studies on the Electrospray Ion Trap Mass Spectra of SFZ-47 and Its Metabolites for a Novel Anti-inflammatory and Analgesic Agent

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (2): 207.

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Studies on the Electrospray Ion Trap Mass Spectra of SFZ-47 and Its Metabolites for a Novel Anti-inflammatory and Analgesic Agent

GU Jing-Kai¹, CHU Da-Feng¹, ZHONG Da-Fang^1,4, LI Yan², ZHOU Hui¹, SHEN Jia-Cong³

1. College of Life Science;
2. Collegeof Chemistry;
3. Key Lab.for Supermolecular Structure and Spectroscopy, Jilin University, Changchun 130023, China;
4. Laboratory of Drug Metabolismand Pharmacokinetics, Shengyang Pharmaceutical University, Shenyang 110015, China

Received:2001-03-15 Online:2002-02-24 Published:2002-02-24

Abstract

Abstract: To investigate the characteristics of mass spectra of 3H-1,2-dihydro-2-(4-methyl-phenylamino)methyl-1-pyrrolizinone(SFZ-47), a novel anti-inflammatory and analgesic agent, and its metabolites, urine samples, which were collected from healthy beagel dogs after a single dose of 200 mg SFZ-47 was taken, were analyzed using electrospray ion trap mass spectrometry in a multi-stage MS full scan mode. The mass spectrometry was operated in the positive mode. The obtained mass spectra were analyzed by assisting with the software Mass Frontier 1.0 for their fragmentation pathways. Besides SFZ-47, four of its metabolites were identified in the urine by LC/MS. The full scan MS₂ or MS₃ spectra of each compound gave characteristic fragment ions of m/z 122 and depyrrolizinone respectively, whereas the ions of dehydration and neutral loss 176 u of two glucuronides were also found. These characteristics can be used for the future structure elucidation in metabolism of SFZ-47 and its analogues.

Key words: 3H-1,2-Dihydro-2-(4-methylphenylamino)methyl-1-pyrrolizinone, Metabolite, Electrospray ion trap mass spectrometer

CLC Number:

R914.1

TrendMD:

GU Jing-Kai, CHU Da-Feng, ZHONG Da-Fang, LI Yan, ZHOU Hui, SHEN Jia-Cong. Studies on the Electrospray Ion Trap Mass Spectra of SFZ-47 and Its Metabolites for a Novel Anti-inflammatory and Analgesic Agent[J]. Chem. J. Chinese Universities, 2002, 23(2): 207.

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Studies on the Electrospray Ion Trap Mass Spectra of SFZ-47 and Its Metabolites for a Novel Anti-inflammatory and Analgesic Agent

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