In-situ Albumin Binding Material for Biomedical Application(Ⅱ)── Albumin Selectivity and in vitro Hemo-compatibility

Abstract

Abstract: In order to assess the hypothesis that the composite structure of srearyl group and PEO might exhibit albumin preferential adsorption by both the albumin bonding affinity of stearyl groups and the protein-resistant character of EO side chains, the adsorption of plasma proteins(fibrinogen and albumin) from buffer and plasma onto poly(methylmethacrylate-co-stearyl-methacrylate)(PMMA-co-SMA), polymethylmethacrylate)-graft-[ω-stearyl-poly(ethylene oxide)](PMMA-g-SPEO) and poly(methyl methacrylate)-graft-[ω-ethyl-poly(ethylene oxide)](PMMA-g-EPEO) was investigated by radioiodine labeling of proteins using the iodine monochloride method. The results confirm that PMMA-g-SPEO can absorb a high level of albumin and resist the fibrinogen adsorption dramatically. The surface for attracting and reversibly binding albumin, which was proved to suppress the platelet adhesion and prolong the clotting time, has been developed on the composite surface structure of srearyl group and PEO.

Key words: Blood compatibility, Protein adsorption, In-situ surface modification, Albumin, Fibrinogen

CLC Number:

O631.3

TrendMD:

JI Jian, FENG Lin-Xian, SHEN Jia-Cong . In-situ Albumin Binding Material for Biomedical Application(Ⅱ)── Albumin Selectivity and in vitro Hemo-compatibility[J]. Chem. J. Chinese Universities, 2002, 23(12): 2369.

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In-situ Albumin Binding Material for Biomedical Application(Ⅱ)── Albumin Selectivity and in vitro Hemo-compatibility

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