Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (5): 744.

• Articles • Previous Articles     Next Articles

DNA-Modified Electrodes(Ⅸ)── Immobilization and Characterization of DNA Probes and the Molecular Hybridization on Gold Surfaces

ZHAO Yuan-Di1, PANG Dai-Wen1, ZHANG Min1, CHENG Jie-Ke1, DAI Hong-Ping2   

  1. 1. Department of Chemistry, Wuhan University, Wuhan 430072, China;
    2. State Key Laboratory for Physical Chemistryof Solid Surfaces, Institute of Physical Chemistry, Department of Chemistry, Xiamen University, Xiamen 361005, China
  • Received:2000-05-11 Online:2001-05-24 Published:2001-05-24

Abstract: Double stranded DNAwas covalently immobilized onto gold surfaces by layer-by-layer immobilization method, and the surfaces were characterized by electrochemical and XPSmethods. The surface molecular hybridization of DNAwas studied on these functionized surfaces. This work might be fundamental for electrochemical gene chips and genosensors for disease diagnosis.

Key words: Gene, Chip, Optimization, Immobilization

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